抗体

抗人类Orai1(细胞外)抗体

ACC-060

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

免疫印迹, 免疫组化, 免疫细胞化学, 免疫组化-石蜡切片

ACC-060

Primary Antibody>Extracellular>Non-Labeled

Insert

抗人类Orai1(细胞外)抗体

Anti-Human Orai1 (extracellular) Antibody (#ACC-060) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry applications. It has been designed to recognize Orai1 from human samples only.

A Rabbit Polyclonal Antibody to Human Orai1 Channel

BLP-CC060

Orai1, TMEM142A, CRACM1, Calcium release-activated calcium channel protein 1

Human Orai1 Channel

Ion Channels > Store-Operated Ca2+ Channels > Antibodies

未共轭

免疫细胞化学, IF, IFC, 免疫组化, LCI, 免疫印迹

兔

多克隆

兔IgG

ORAI1

人类(试验)

Canis - identical; bovine - 12/13 amino acid residues identical.

Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling intracellular Ca2+ level influx either by the calcium-release-activated Ca2+ channels (CRAC), or from intracellular stores, has become of great interest. Recently, several key players of the store operated complex have been identified3. Orai1 (also known as CRACM1) acts as the store operated Ca2+ channel (SOC) and STIM1, which acts as the endoplasmic reticulum Ca2+ sensor3,4. The formation of functional channels requires the presence of both Orai1 and STIM1 proteins working as a complex and involves the co-clustering of Orai1 on the plasma membrane with STIM1 on the endoplasmic reticulum4-6. TRPC1, a member of the transient receptor potential family was also suggested to act as a player in the SOC complex7. In T-cells, Ca2+ entry following activation by antigen-receptor engagement occurs solely through CRAC channels where Orai1 constitutes the pore forming subunit3,8. Orai1 is a plasma membrane protein with four potential transmembrane domains and intracellular N- and C-terminus. In addition, two mammalian homologs to Orai1 have been identified; Orai2 and Orai33,9. All three, Orai1 Orai2 and Orai3, are capable of forming store operated channels with different magnitudes9.

合成肽

细胞外

2nd extracellular loop

(C)KKQPGQPRPTSK, corresponding to amino acid residues 203-214 of human Orai1

Peptide (C)KKQPGQPRPTSK, corresponding to amino acid residues 203-214 of human Orai1 (Accession Q96D31). 2nd extracellular loop.

1 X 25 mcl

0.65毫克/毫升

065毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

Q96D31

ACC-063, ACC-061, ACC-010, ACC-065, AGP-005

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元

Cell surface detection of Orai1 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells: Cell surface detection of Orai1 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells: Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells + Anti-Human Orai1 (extracellular) Antibody (#ACC-060), (5μg) + goat-anti-rabbit-FITC.