产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗TRPC6(细胞外)抗体
目录 :
ACC-120
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
文章摘录数: 14
出版应用/物种/样本/稀释 | 参考文献 |
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图像
图像 1 :
Western blot analysis of rat brain lysates: - 1. Anti-TRPC6 (extracellular) Antibody (#ACC-120), (1:200).2. Anti-TRPC6 (extracellular) Antibody, preincubated with TRPC6 (extracellular) Blocking Peptide (#BLP-CC120).
图像 2 :
Expression of TRPC6 in rat PC12 cells - Cell surface detection ofTRPC6 in intact living PC12 cells. A. Extracellular staining of cells with Anti-TRPC6 (extracellular)Antibody (#ACC-120) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining with DAPI as the counterstain. C. Merged image of A and B.
图像 3 :
TRPC6 is required for stretch activation in myocytes. - Electrophysiological recordings of myocytes incubated with Anti-TRPC6 (extracellular) Antibody (#ACC-120) in the absence (upper left) and in the presence (lower left) of the control peptide antigen. Immunocytochemical staining of living rat cerebral artery smooth muscle cells using Anti-TRPC6 (extracellular) antibody. Preincubation with the control peptide antigen blocks TRPC6 immunostaining (lower right panel).Adapted from Gonzales A.L. et al. with permission of the American Association for the Advancement of Science.
产品信息
SKU号 :
ACC-120_1_25_mcl
ParentSKU :
ACC-120
Taxonomy Node :
Primary Antibody>Extracellular>Non-Labeled
Action :
Insert
产品名称 :
抗TRPC6(细胞外)抗体
产品描述 :
Anti-TRPC6 (extracellular) Antibody (#ACC-120) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry and live cell flow cytometry applications. It has been designed to recognize TRPC6 from rat, mouse and human samples.
Short Description :
A Rabbit Polyclonal Antibody to TRPC6 Channel
Negative Control :
BLP-CC120
同义词 :
TRPC6, Short transient receptor potential channel 6, Transient receptor protein 6, TRP6
靶标名 :
TRPC6 Channel
Primary Category (Old) :
Ion Channels > TRP Channels > TRPC Channels > Antibodies to TRPC Channels
标记/共轭标签 :
未共轭
应用 :
免疫细胞化学, IF, IFC, 免疫组化, LCI, 免疫印迹
宿主 :
兔
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
TRPC6
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Rat, human - identical
靶标/背景信息 :
The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels1-4.
The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: TRPC1, TRPC4, TRPC5 TRPC3, TRPC6, TRPC7 and TRPC22,5. They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.
TRPC6 can form heterotetramers with TRPC3 and TRPC7. It is primarily expressed in brain, lung and muscle. High levels of expression of the channel were also found in human platelets. Recently it was reported that TRPC6 is also expressed in the kidney where a mutated channel has been implicated in kidney failure disease6,7.
免疫原 :
合成肽
区域 :
细胞外
Loop :
2nd extracellular loop
序列 :
(C)DNVKYYNLARIKWD, corresponding to amino acid residues 573-586 of rat TRPC6
序列文本 :
Peptide (C)DNVKYYNLARIKWD, corresponding to amino acid residues 573-586 of rat TRPC6 (Accession Q61143). 2nd extracellular loop.
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.85毫克/毫升
浓度 :
085毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
Q61143
Top Related :
ACC-017, ACC-016, ACC-118, ACC-049, ACC-010
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
IFC Legend :
Cell surface detection of TRPC6 by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: Cell surface detection of TRPC6 by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells + Anti-TRPC6 (extracellular) Antibody (#ACC-120), (2.5μg) + goat-anti-rabbit-FITC.
LCI, ICC Legend :
Expression of TRPC6 in rat PC12 cells - Cell surface detection of TRPC6 in intact living PC12 cells. A. Extracellular staining of cells with Anti-TRPC6 (extracellular) Antibody (#ACC-120), (1:50), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Nuclear staining with DAPI as the counterstain. C. Merged image of A and B.
更多信息或购买 :
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年,在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。