抗体

抗连接蛋白抗体

ACC-201

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

ACC-201

Primary Antibody>Intracellular>Non-Labeled

Insert

抗连接蛋白抗体

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of human Connexin-43. Anti-Connexin-43 Antibody (#ACC-201) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize Cx43 from human, rat and mouse samples.

A兔多克隆抗体连接蛋白

BLP-CC201

Connexin-43, CX43, Gap junction alpha1 protein, GJA1, GJAL, Gap junction 43 kDa heart protein

连接蛋白

Ion Channels > Gap Junction Channels > Connexin Family > Antibodies

未共轭

免疫细胞化学, IF, 免疫组化, 免疫印迹

兔

多克隆

兔IgG

GJA1

人类(试验);小鼠(试验);大鼠(试验)

Rat - 14/15 amino acid residues identical; mouse - 13/15 amino acid residues identical.

Connexins (Cx) are integral membrane proteins consisting of four transmembrane domains, two extracellular loops, one intracellular loop and intracellular N- and C-termini. The 21 members belonging to this family form homomeric or heteromeric hexamers generally termed connexons or hemi-channels. In turn, these hemi-channels further assemble in a head-to-head manner, thus forming gap junction channels1,2. Connexins are ubiquitously expressed and their activity is regulated at the expression level and by post-translational modifications1. For example, Connexin-43 (Cx43) protein level is regulated by its turnover rate and by phosphorylation of various residues which ultimately determines its activity rate1. Gap junctions are usually found in clusters and enable intercellular communication by allowing the passage of small molecules between cells3. They play important roles in different biological processes. These include differentiation, cell cycle synchronization, cellular development, neuronal activity and the immune response2,4,5. Due to their important roles, mutations in connexins are linked with a number of diseases such as neurodegenerative disorders, skin diseases, deafness, and developmental abnormalities2,5,6.

合成肽

intracellular

Intracellular, C-terminus

(C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 of human Connexin-43

Peptide (C)HAQPFDFPDDNQNSK, corresponding to amino acids residues 331-345 of human Connexin-43 (Accession P17302). Intracellular, C-terminus.

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P17302

APC-052, ACC-209, ACC-234, ARR-002, APC-022

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元

Expression of Connexin-43 in rat intestinal epithelial IEC-6 cells - Immunocytochemical staining of fixed and permeabilized rat intestinal epithelial IEC-6 cells. Cells were stained with Anti-Connexin-43 Antibody (#ACC-201), (1:200), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). Cell nuclei were visualized using Hoechst 33342 (blue).