产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗趋化因子受体4(细胞外)抗体
目录 :
ACR-014
规格 :
1 X 50 mcl
价格 :
530美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
文章摘录数: 6
参考文献
Albee L, Gao X, Majetschak M. Plasticity of seven-transmembrane-helix receptor heteromers in human vascular smooth muscle cells. PLoS ONE. 2021;16:e0253821 pubmed 出版商
Chen C, Chen Y, Li M, Xiao H, Shi Q, Zhang T, et al. Functional decellularized fibrocartilaginous matrix graft for rotator cuff enthesis regeneration: A novel technique to avoid in-vitro loading of cells. Biomaterials. 2020;250:119996 pubmed 出版商
Ciullo A, Biemmi V, Milano G, Bolis S, Cervio E, Fertig E, et al. Exosomal Expression of CXCR4 Targets Cardioprotective Vesicles to Myocardial Infarction and Improves Outcome after Systemic Administration. Int J Mol Sci. 2019;20: pubmed 出版商
Gao X, Abdelkarim H, Albee L, Volkman B, Gaponenko V, Majetschak M. Partial agonist activity of α1-adrenergic receptor antagonists for chemokine (C-X-C motif) receptor 4 and atypical chemokine receptor 3. PLoS ONE. 2018;13:e0204041 pubmed 出版商
Gao X, Albee L, Volkman B, Gaponenko V, Majetschak M. Asymmetrical ligand-induced cross-regulation of chemokine (C-X-C motif) receptor 4 by α1-adrenergic receptors at the heteromeric receptor complex. Sci Rep. 2018;8:2730 pubmed 出版商
Albee L, Eby J, Tripathi A, Laporte H, Gao X, Volkman B, et al. ?1-Adrenergic Receptors Function Within Hetero-Oligomeric Complexes With Atypical Chemokine Receptor 3 and Chemokine (C-X-C motif) Receptor 4 in Vascular Smooth Muscle Cells. J Am Heart Assoc. 2017;6: pubmed 出版商
图像
图像 1 :
Alomone Labs ACR-014 图像 1
Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-CXCR4 (extracellular) Antibody (#ACR-014), (1:200).3,4. Anti-CXCR4 (extracellular) Antibody, preincubated with CXCR4 (extracellular) Blocking Peptide (#BLP-CR014).
图像 2 :
Alomone Labs ACR-014 图像 2
Expression of CXCR4 in rat cerebellum - Immunohistochemical staining of rat cerebellum frozen section using Anti-CXCR4 (extracellular) Antibody (#ACR-014), (1:100). CXCR4 is expressed in Purkinje cell bodies and axonal prolongations (arrows). Hoechst 33342 is used as the counterstain.
图像 3 :
Alomone Labs ACR-014 图像 3
Cell surface detection of CXCR4 in Jurkat living cells: - ___Unstained cells.___Cells +Anti-CXCR4 (extracellular)Antibody (#ACR-014) (10 g/0.5x106cells).
产品信息
SKU号 :
ACR-014_1_50_mcl
ParentSKU :
ACR-014
Taxonomy Node :
Primary Antibody>Extracellular>Non-Labeled
Action :
Insert
产品名称 :
抗趋化因子受体4(细胞外)抗体
产品描述 :
Anti-CXCR4 (extracellular) Antibody (#ACR-014) is a highly specific antibody directed against an extracellular epitope of the human CXCR4 chemokine receptor. The antibody can be used for western blot, immunocytochemistry, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize CXCR4 from rat, mouse and human samples.
Short Description :
A兔多克隆抗体趋化因子受体4趋化因子受体
Negative Control :
BLP-CR014
同义词 :
CXCR4, C-X-C chemokine receptor type 4, CD184, Fusin, LESTR, NPYRL, SDF-1 receptor, WHIMS
靶标名 :
趋化因子受体4趋化因子受体
Primary Category (Old) :
G-Protein Coupled Receptors > Chemokine Receptors > Antibodies
标记/共轭标签 :
未共轭
应用 :
免疫细胞化学, IF, IFC, 免疫组化, LCI, 免疫印迹
宿主 :
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
趋化因子受体4
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Mouse - 12/14 amino acid residues identical; rat - 9/14 amino acid residues identical.
靶标/背景信息 :
Chemokines are small molecular weight, soluble secreted proteins that bind and activate their respective G-protein coupled receptor (GPCR), chemokine receptors in order to evoke a cellular response resulting in migration or chemotaxis1. The chemokine system involves more than 40 chemokines and 18 chemokine receptors. The receptors are designated CXCR1-5, CCR1-11, XCR1 and CX3CR1, based on their specific ligand preference2. Chemokine receptors are present on many different cell types. They were initially detected on leukocytes, where they were found to play an important role in the migration of these cells to inflammation sites3. CXCR4 was originally identified as an orphan receptor, and soon gained much attention when it was discovered as a coreceptor for HIV-14. Besides from being involved in HIV-1 infection/progression, CXCR4 is found to be upregulated in many different cancers/tumors and has evolved to become a target for the development of antagonists5. CXCL12 (SDF-1α) is the sole ligand for CXCR4. Following binding of its ligand, CXCR4 undergoes dimerization and activates Gi G-proteins6,7. However downstream activation through CXCR4 could also occur through other G-proteins and non-G-proteins5. The down regulation of the CXCR4 receptor is initiated by phosphorylation of its cytoplasmic tail, which is followed by the binding of arrestin. The receptor is then internalized through endocytosis and degraded in the lysosome. Downregulation of CXCR4 could also occur through the stimulation of other GPCRs8. The distribution of CXCR4 is quite broad and involves the central nervous system (CNS)9, spleen10, testes11, hematopoietic and non-hematopoietic cells12.
免疫原 :
合成肽
区域 :
细胞外
Loop :
细胞外, N末端
序列 :
(C)EGISIYTSDNYTEE, corresponding to amino acid residues 2-15 of human CXCR4
序列文本 :
Peptide (C)EGISIYTSDNYTEE, corresponding to amino acid residues 2-15 of human CXCR4 (Accession P61073). Extracellular, N-terminus.
Primary Size (Size+Units) :
1 X 50 mcl
浓度 :
0.8毫克/毫升
浓度 :
08毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P61073
储存和重组 :
Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
包装规格 :
1
产品规格/规格 :
50
单位 :
mcl
标价美国[美元] :
530美元
IFC Legend :
Cell surface detection of CXCR4 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:  Cell surface detection of CXCR4 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells:  Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells +Anti-CXCR4 (extracellular) Antibody (#ACR-014), (2.5μg) + goat-anti-rabbit-FITC.
更多信息或购买 :
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年,在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。