抗体

抗人类CCR3(细胞外)抗体

ACR-023

1 X 50 mcl

530美元

多克隆

domestic rabbit

未共轭

ACR-023

Primary Antibody>Extracellular>Non-Labeled

Insert

抗人类CCR3(细胞外)抗体

Anti-Human CCR3 (extracellular) Antibody (#ACR-023) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize CCR3 from human samples only. The antibody will not recognize the receptor from mouse or rat samples.

A兔多克隆抗体CCR3趋化因子受体

BLP-CR023

CCR3, C-C chemokine receptor type 3, Eosinophil eotaxin receptor, CKR3, C-C CKR-3, CMKBR3, B-chemokine receptor, CD193 antigen

CCR3趋化因子受体

G-Protein Coupled Receptors > Chemokine Receptors > Antibodies

未共轭

IFC, LCI, 免疫印迹

兔

多克隆

兔IgG

CCR3

人类(试验)

人类只有

CCR3 (C-C chemokine receptor type 3) is a seven transmembrane G-protein coupled receptor (GPCR) that acts as a key regulator in the homeostasis of the immune system. The receptor is responsible for recruiting eosinophils to local inflammatory tissues. CCR3 is the endogenous receptor for Eotaxin, C-C motif chemokine 11 (CCL11). The binding between CCR3 and CCL11 activates G-protein-dependent signaling pathways and thus enables the release of eosinophils progenitor cells and mature eosinophils to the affected tissue. Studies have shown that deficiency in CCR3 protein may reduce the binding of Eotaxin and inhibition of phosphatidylinositol-3-kinase (PI3K) signaling pathway activation and the inactivation of its subunit PI3Kγ1. Other CCR3 ligands include CCL5, CCL24, CCL26, CCL8, CCL7, and CCL121. CCR3 is linked to several type-I allergy diseases, including allergic rhinitis. In addition to CCR3 expression in eosinophils, CCR3 is also expressed in basophils, microglia, astrocytes and neurons in both the developing and adult brain1,2. The expression of CCR3 increases following human immunodeficiency virus infection and in reactive microglia and astrocytes around the amyloid deposits in Alzheimer’s disease2.

合成肽

细胞外

2nd extracellular loop

CSALYPEDTVYSWRH, corresponding to amino acid residues 183-197 of human CCR3

Peptide CSALYPEDTVYSWRH, corresponding to amino acid residues 183-197 of human CCR3 (Accession P51677). 2nd Extracellular loop.

1 X 50 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P51677

Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

50

mcl

530美元

Cell surface detection of CCR3 in live intact human Jurkat T cell leukemia cells: Cell surface detection of CCR3 in live intact human Jurkat T cell leukemia cells: Black line: Cells. Red line: Cells + goat-anti-rabbit-Alexa-488. Green line: Cells + Anti-Human CCR3 (extracellular) Antibody (#ACR-023), (5 µg/1x106 cells) + goat-anti-rabbit-Alexa-488.