抗体

抗EMR1(ADGRE1)(细胞外)抗体

AER-051

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫细胞化学

AER-051

Primary Antibody>Extracellular>Non-Labeled

Insert

抗EMR1(ADGRE1)(细胞外)抗体

Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell imaging applications. It recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize EMR1 from human, mouse, and rat samples.

A兔多克隆抗体EMR1

BLP-ER051

EMR1 (ADGRE1), EMR1 hormone receptor, EGF-like module-containing mucin-like hormone receptor-like 1, Adhesion G protein-coupled receptor E1, TM7LN3, F4/80, GPF480

EMR1

G-Protein Coupled Receptors > Adhesion Class GPCRs > Antibodies

未共轭

免疫细胞化学, IF, IFC, LCI, 免疫印迹

兔

多克隆

兔IgG

ADGRE1

人类(试验);小鼠(试验);大鼠(试验)

Mouse, Rat –11/14 amino acid residues identical.

Human epidermal growth factor (EGF)–like module containing mucin-like hormone receptor 1 (EMR1) is a surface receptor with unknown function that belongs to the adhesion family of the G protein-coupled receptors (GPCR). This receptor is encoded by the ADGRE1 and like all GPCRs, contains seven transmembrane domains, an intracellular C-terminus and an extracellular N-terminus. The NH2 domain has six EGF-like domains. Adhesion GPCRs share a long N-terminal extracellular sequences comprising multiple domains, separated from the transmembrane segments by a serine/threonine-rich domain, a feature reminiscent of mucin-like, single-span, integral membrane glycoproteins with adhesive properties1,2. EMR1 protein is a homolog of F4/80, a murine protein that is widely being used as a marker of murine macrophage populations1. Human EMR1 is exclusively expressed on mature eosinophils in the blood and bone marrow and in nasal polyps. Thus, EMR1 is considered to be a potential therapeutic target for the treatment of eosinophilic disorders1,3.

合成肽

细胞外

细胞外, N末端

CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1

Peptide CKQGFLSSNGQNHFK, corresponding to amino acid residues 58-72 of human EMR1 (Accession Q14246). Extracellular, N-terminus.

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

Q14246

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元

Cell surface detection of EMR1 (F4/80) by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: Cell surface detection of EMR1 (F4/80) by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells +  Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051), (2.5μg) + goat-anti-rabbit-FITC.

Expression of EMR1 in rat RBL cells - Cell surface detection of EMR1 in intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-EMR1 (ADGRE1) (extracellular) Antibody (#AER-051), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red), (1:100). B. Live view of the cells. C. Merged image of panels A and B.