抗体

Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody

AGC-002-AG

1 X 50 mcl

735美元

多克隆

domestic rabbit

ATTO 488

人类, 小鼠, 大鼠

免疫组化, 免疫细胞化学

AGC-002-AG

Primary Antibody>Extracellular>Labeled

Insert

Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody

Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry. It has been designed to recognize GluN2A from rat, mouse, and human samples. nAnti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody (#AGC-002-AG) is directly labeled with an ATTO-488 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-488 label is analogous to the well known dye fluorescein isothiocyanate (FITC) and can be used with filters typically used to detect FITC. Anti-NMDAR2A (GluN2A) (extracellular)-ATTO Fluor-488 Antibody has been tested in immunohistochemistry and is especially suited for experiments requiring simultaneous labeling of different markers.

A Rabbit Polyclonal Antibody to NMDA Receptor 2A (GluN2A) Conjugated to the Fluorescent Dye ATTO-488

BLP-GC002

NMDAR2A (GluN2A), NMDA receptor 2A, Ionotropic glutamate receptor subunit epsilon1, N-methyl-D-aspartate receptor subunit 2A, GRIN2A, GLUN2A, NR2A

Ion Channels > Ligand-Gated Ion Channels > Glutamate Receptors > NMDA Receptors > Antibodies to NMDA Receptors

ATTO 488 (Green)

IF, 免疫组化

兔

多克隆

兔IgG

GRIN2A

人类(试验);小鼠(试验);大鼠(试验)

Human, mouse - identical.

The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors. The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit. NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels. Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson’s and Alzheimer’s disease. NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

合成肽

细胞外

细胞外, N末端

GHSHDVTERELRN(C), corresponding to amino acid residues 41-53 of rat NR2A

Peptide GHSHDVTERELRN(C), corresponding to amino acid residues 41-53 of rat NR2A (Accession Q00959). Extracellular, N-terminus.

1 X 50 mcl

1毫克/毫升

1毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

Q00959

AGC-002

Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

50

mcl

735美元