抗NMDAR2A(GluN2A)(细胞外)抗体 | Alomone Labs AGC-002 产品信息

产品简要

公司名称 :

Alomone Labs

产品类型 :

抗体

产品名称 :

抗NMDAR2A(GluN2A)(细胞外)抗体

AGC-002

规格 :

1 X 25 mcl

价格 :

425美元

克隆性 :

多克隆

宿主 :

domestic rabbit

共轭标签 :

未共轭

反应物种 :

人类, 小鼠, 大鼠

应用 :

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-自由浮动切片, 其他

更多信息或购买 :

Alomone Labs 产品网页

文章摘录数: 16

出版应用/物种/样本/稀释	参考文献
免疫组化; 人类; 1:1000; 图 2c	Dong Y, Hsu F, Koziol White C, Stepanova V, Jude J, Gritsiuta A, et al. Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells. Sci Rep. 2021;11:8205 pubmed 出版商
其他; 小鼠; 1:200; 图 1b	Al Abed A, Sellami A, Potier M, Ducourneau E, Gerbeaud Lassau P, Brayda Bruno L, et al. Age-related impairment of declarative memory: linking memorization of temporal associations to GluN2B redistribution in dorsal CA1. Aging Cell. 2020;19:e13243 pubmed 出版商
免疫组化-自由浮动切片; 小鼠; 1:500; 图 8e	Yeung J, Palpagama T, Tate W, Peppercorn K, Waldvogel H, Faull R, et al. The Acute Effects of Amyloid-Beta1-42 on Glutamatergic Receptor and Transporter Expression in the Mouse Hippocampus. Front Neurosci. 2019;13:1427 pubmed 出版商
免疫细胞化学; 小鼠; 1:100; 图 s2	Roshanravan H, Kim E, Dryer S. NMDA Receptors as Potential Therapeutic Targets in Diabetic Nephropathy: Increased Renal NMDA Receptor Subunit Expression in Akita Mice and Reduced Nephropathy Following Sustained Treatment With Memantine or MK-801. Diabetes. 2016;65:3139-50 pubmed 出版商
	Tseng W, Reinhart V, Lanz T, Weber M, Pang J, Le K, et al. Schizophrenia-associated SLC39A8 polymorphism is a loss-of-function allele altering glutamate receptor and innate immune signaling. Transl Psychiatry. 2021;11:136 pubmed 出版商
	Johansson E, Bouchet D, Tamouza R, Ellul P, Morr A, Avignone E, et al. Human endogenous retroviral protein triggers deficit in glutamate synapse maturation and behaviors associated with psychosis. Sci Adv. 2020;6:eabc0708 pubmed 出版商
	Bayraktar G, Yuanxiang P, Confettura A, Gomes G, Raza S, Stork O, et al. Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus. Neuropsychopharmacology. 2020;45:2120-2130 pubmed 出版商
	Carbonell A, Cho C, Tindi J, Counts P, Bates J, Erdjument Bromage H, et al. Haploinsufficiency in the ANKS1B gene encoding AIDA-1 leads to a neurodevelopmental syndrome. Nat Commun. 2019;10:3529 pubmed 出版商
	Li L, Yin N, Li X, Miao Y, Cheng S, Li F, et al. Rac1 Modulates Excitatory Synaptic Transmission in Mouse Retinal Ganglion Cells. Neurosci Bull. 2019;: pubmed 出版商
	Tan C, Lu N, Wang C, Chen D, Sun N, Lyu H, et al. Endothelium-Derived Semaphorin 3G Regulates Hippocampal Synaptic Structure and Plasticity via Neuropilin-2/PlexinA4. Neuron. 2019;101:920-937.e13 pubmed 出版商
	Rocchio F, Tapella L, Manfredi M, Chisari M, Ronco F, Ruffinatti F, et al. Gene expression, proteome and calcium signaling alterations in immortalized hippocampal astrocytes from an Alzheimer's disease mouse model. Cell Death Dis. 2019;10:24 pubmed 出版商
	Fan Y, Guan S, Luo L, Li Y, Yang L, Zhou X, et al. Tetrahydroxystilbene glucoside relieves the chronic inflammatory pain by inhibiting neuronal apoptosis, microglia activation, and GluN2B overexpression in anterior cingulate cortex. Mol Pain. 2018;14:1744806918814367 pubmed 出版商
	Li Y, Kim R, Cho Y, Song W, Kim D, Kim K, et al. Lrfn2-Mutant Mice Display Suppressed Synaptic Plasticity and Inhibitory Synapse Development and Abnormal Social Communication and Startle Response. J Neurosci. 2018;38:5872-5887 pubmed 出版商
	Sibarov D, Bruneau N, Antonov S, Szepetowski P, Burnashev N, Giniatullin R. Functional Properties of Human NMDA Receptors Associated with Epilepsy-Related Mutations of GluN2A Subunit. Front Cell Neurosci. 2017;11:155 pubmed 出版商
	Konstantoudaki X, Chalkiadaki K, Tivodar S, Karagogeos D, Sidiropoulou K. Impaired synaptic plasticity in the prefrontal cortex of mice with developmentally decreased number of interneurons. Neuroscience. 2016;322:333-45 pubmed 出版商
	Telezhkin V, Schnell C, Yarova P, Yung S, Cope E, Hughes A, et al. Forced cell cycle exit and modulation of GABAA, CREB, and GSK3Î² signaling promote functional maturation of induced pluripotent stem cell-derived neurons. Am J Physiol Cell Physiol. 2016;310:C520-41 pubmed 出版商

图像

图像 1 :

Western blot analysis of rat brain lysates: - 1. Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002), (1:600).2. Anti-NMDAR2A (GluN2A) (extracellular) Antibody, preincubated with NMDAR2A/GluN2A (extracellular) Blocking Peptide (#BLP-GC002).

图像 2 :

Multiplex staining of GluN2A and GluN2B in mouse deep cerebellar nucleus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (#AGC-003-AR), (1:60) and Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002), (1:200). A. Sections were incubated with Anti-NMDAR2A (GluN2A) (extracellular) Antibody, followed by goat anti-rabbit-Alexa-488 (green). B. The same sections were incubated with Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (red). C. Merge of A and B demonstrates the ubiquitous colocalization of the GluN2A and GluN2B subunits in cells with neuronal profiles in this nucleus. Arrows point at an example of NR2A and NR2B co-expression.

产品信息

SKU号 :

AGC-002_1_25_mcl

ParentSKU :

AGC-002

Taxonomy Node :

Primary Antibody>Extracellular>Non-Labeled

Action :

Insert

产品名称 :

抗NMDAR2A(GluN2A)(细胞外)抗体

产品描述 :

Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry. The antibody recognizes an extracellular epitope and is thus ideal for detecting GluN2A in living cells. It has been designed to recognize GluN2A from rat, mouse, and human samples.

Short Description :

A兔多克隆抗体 NMDA受体2A(GluN2A)

Negative Control :

BLP-GC002

同义词 :

NMDAR2A (GluN2A), NMDA receptor 2A, Ionotropic glutamate receptor subunit epsilon1, N-methyl-D-aspartate receptor subunit 2A, GRIN2A, GLUN2A, NR2A

Primary Category (Old) :

Ion Channels > Ligand-Gated Ion Channels > Glutamate Receptors > NMDA Receptors > Antibodies to NMDA Receptors

标记/共轭标签 :

未共轭

应用 :

免疫细胞化学, IF, 免疫组化, 免疫沉淀, LCI, 免疫印迹

宿主 :

兔

克隆性 :

多克隆

抗体亚型 :

兔IgG

基因符号 :

GRIN2A

反应物种 :

人类(试验);小鼠(试验);大鼠(试验)

同源 :

Human, mouse - identical.

靶标/背景信息 :

The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors. The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit. The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels. Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson’s and Alzheimer’s disease. NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

免疫原 :

合成肽

区域 :

细胞外

Loop :

细胞外, N末端

序列 :

GHSHDVTERELRN(C), corresponding to amino acid residues 41-53 of rat NR2A

序列文本 :

Peptide GHSHDVTERELRN(C), corresponding to amino acid residues 41-53 of rat NR2A (Accession Q00959). Extracellular, N-terminus.

Primary Size (Size+Units) :

1 X 25 mcl

浓度 :

0.55毫克/毫升

浓度 :

055毫克/毫升

缓冲液 :

磷酸盐缓冲液(PBS)

pH值 :

pH值7.4

防腐剂 :

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Additive(s) :

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

格式(液体/冻干) :

冻干粉末

纯化来源(杂交瘤细胞, 腹水 :

Affinity purified on immobilized antigen

储存温度 :

-20°C

UniProt数据库 :

Q00959

Top Related :

AGC-003, AGC-001, AGC-004, AGP-046, AGC-005

储存和重组 :

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

包装规格 :

产品规格/规格 :

单位 :

mcl

标价美国[美元] :

425美元

LCI, ICC Legend :

Expression of NR2A in rat C6 glioma cells - Cell surface detection of NR2A in live intact rat C6 glioma cells. A. Cells were stained with Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002), (1:100), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). Cell nuclei were stained with the cell permeable dye Hoechst 33342 (blue staining). B. Live view of the same field.

IP Legend :

Immunoprecipitation of rat brain lysates - 1. Cell lysate.2. Cell lysates + protein A beads + Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002).3. Cell lysates + protein A beads + pre-immune rabbit serum. Black arrow indicates the NR2A protein while the red arrow shows the IgG heavy chain. Immunoblot was performed with Anti-NMDAR2A (GluN2A) (extracellular) Antibody.

更多信息或购买 :

Alomone Labs 产品网页

公司信息

Alomone Labs

Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201

info@alomone.com

http://www.alomone.com

972-2-587-2202

公司总部: 以色列

Alomone Labs公司成立于1989年，在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。