产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody
目录 :
AGC-003-AR
规格 :
1 X 50 mcl
价格 :
735美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
ATTO 594
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫组化, 免疫细胞化学
更多信息或购买 :
文章摘录数: 4
参考文献
Espana A, Seth H, J xe9 z xe9 quel J, Huang T, Bouchet D, Lepleux M, et al. Alteration of NMDA receptor trafficking as a cellular hallmark of psychosis. Transl Psychiatry. 2021;11:444 pubmed 出版商
Johansson E, Bouchet D, Tamouza R, Ellul P, Morr A, Avignone E, et al. Human endogenous retroviral protein triggers deficit in glutamate synapse maturation and behaviors associated with psychosis. Sci Adv. 2020;6:eabc0708 pubmed 出版商
Bland T, Zhu M, Dillon C, Sahin G, Rodriguez Llamas J, Appleyard S, et al. Leptin Controls Glutamatergic Synaptogenesis and NMDA-Receptor Trafficking via Fyn Kinase Regulation of NR2B. Endocrinology. 2020;161: pubmed 出版商
Li L, Yin N, Li X, Miao Y, Cheng S, Li F, et al. Rac1 Modulates Excitatory Synaptic Transmission in Mouse Retinal Ganglion Cells. Neurosci Bull. 2019;: pubmed 出版商
图像
图像 1 :
Alomone Labs AGC-003-AR 图像 1
Multiplex staining of GluN2A and GluN2B in mouse deep cerebellar nucleus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (#AGC-003-AR), (1:60) and Anti-NMDAR2A (GluN2A) (extracellular) Antibody (#AGC-002), (1:200). A. Sections were incubated with Anti-NMDAR2A (GluN2A) (extracellular) Antibody, followed by goat anti-rabbit-Alexa-488 (green). B. The same sections were incubated with Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (red). C. Merge of A and B demonstrates the ubiquitous colocalization of the GluN2A and GluN2B subunits in cells with neuronal profiles in this nucleus.  Arrows point at an example of NR2A and NR2B co-expression.
产品信息
SKU号 :
AGC-003-AR_1_50_mcl
ParentSKU :
AGC-003-AR
Taxonomy Node :
Primary Antibody>Extracellular>Labeled
Action :
Insert
产品名称 :
Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody
产品描述 :
Anti-NMDAR2B (GluN2B) (extracellular) Antibody (#AGC-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, live cell imaging, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize GluN2B from human, rat, and mouse samples. nAnti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody (#AGC-003-AR) is directly labeled with an ATTO-594 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. The ATTO-594 fluorescent label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. Anti-NMDAR2B (GluN2B) (extracellular)-ATTO Fluor-594 Antibody has been tested in immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers.
Short Description :
A Rabbit Polyclonal Antibody to NMDA Receptor 2B (GluN2B) Conjugated to the Fluorescent Dye ATTO-594
Negative Control :
BLP-GC003
同义词 :
NMDAR2B (GluN2B), NMDA receptor 2B, Ionotropic glutamate receptor subunit epsilon2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B
Primary Category (Old) :
Ion Channels > Ligand-Gated Ion Channels > Glutamate Receptors > NMDA Receptors > Antibodies to NMDA Receptors
标记/共轭标签 :
ATTO 594 (Red)
应用 :
IF, 免疫组化
宿主 :
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
GRIN2B
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Human, mouse - identical.
靶标/背景信息 :
The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors. The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit. The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels. Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson’s and Alzheimer’s diseases. NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.
免疫原 :
合成肽
区域 :
细胞外
Loop :
细胞外, N末端
序列 :
(C)NTHEKRIYQSNMLNR, corresponding to amino acid residues 323-337 of rat NMDAR2B
序列文本 :
Peptide (C)NTHEKRIYQSNMLNR, corresponding to amino acid residues 323-337 of rat NMDAR2B (Accession Q00960). Extracellular, N-terminus.
Primary Size (Size+Units) :
1 X 50 mcl
浓度 :
1毫克/毫升
浓度 :
1毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
Q00960
Top Related :
AGP-046, AGC-003, AGC-002-AG
储存和重组 :
Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
50
单位 :
mcl
标价美国[美元] :
735美元
更多信息或购买 :
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年,在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。