抗体

抗NMDAR2B(GluN2B)(细胞外)抗体

AGC-003

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

AGC-003

Primary Antibody>Extracellular>Non-Labeled

Insert

抗NMDAR2B(GluN2B)(细胞外)抗体

Anti-NMDAR2B (GluN2B) (extracellular) Antibody (#AGC-003) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, live cell imaging, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize GluN2B from human, rat, and mouse samples.

A兔多克隆抗体 NMDA受体2B(GluN2B)

BLP-GC003

NMDAR2B (GluN2B), NMDA receptor 2B, Ionotropic glutamate receptor subunit epsilon2, N-methyl-D-aspartate receptor subunit 2B, GRIN2B, NR2B

Ion Channels > Ligand-Gated Ion Channels > Glutamate Receptors > NMDA Receptors > Antibodies to NMDA Receptors

未共轭

免疫细胞化学, IF, 免疫组化, 免疫沉淀, LCI, 免疫印迹

兔

多克隆

兔IgG

GRIN2B

人类(试验);小鼠(试验);大鼠(试验)

Human, mouse - identical.

The NMDA receptors are members of the glutamate receptor family of ion channels that also include the AMPA and Kainate receptors. The NMDA receptors are encoded by seven genes: one NMDAR1 (or NR1) subunit, four NR2 (NR2A-NR2D) and two NR3 (NR3A-NR3B) subunits. The functional NMDA receptor appears to be a heterotetramer composed of two NMDAR1 and two NMDAR2 subunits. Whereas the NMDAR2 subunits that assemble with the NMDAR1 subunit can be either of the same kind (i.e. two NMDAR2A subunits) or different (one NMDAR2A with one NMDAR2B). NMDAR3 subunits can substitute the NMDAR2 subunits in their complex with the NMDAR1 subunit. The NMDAR is unique among ligand-gated ion channels in that it requires the simultaneous binding of two obligatory agonists: glycine and glutamate that bind to the NMDAR1 and NMDAR2 binding sites respectively. Another unique characteristic of the NMDA receptors is their dependence on membrane potential. At resting membrane potentials the channels are blocked by extracellular Mg2+. Neuronal depolarization relieves the Mg2+ blockage and allows ion influx into the cells. NMDA receptors are strongly selective for Ca2+ influx differing from the other glutamate receptor ion channels that are non-selective cation channels. Ca2+ entry through the NMDAR regulates numerous downstream signaling pathways including long term potentiation (a molecular model of memory) and synaptic plasticity that may underlie learning. In addition, the NMDA receptors have been implicated in a variety of neurological disorders including epilepsy, ischemic brain damage, Parkinson’s and Alzheimer’s disease. NMDA receptors expression and function are modulated by a variety of factors including receptor trafficking to the synapses and internalization as well as phosphorylation and interaction with other intracellular proteins.

合成肽

细胞外

细胞外, N末端

(C)NTHEKRIYQSNMLNR, corresponding to amino acid residues 323-337 of rat NMDA receptor 2B

Peptide (C)NTHEKRIYQSNMLNR, corresponding to amino acid residues 323-337 of rat NMDA receptor 2B (Accession Q00960). Extracellular, N-terminus.

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

Q00960

AGC-002, AGC-001, AGP-046, AGC-031, AGC-020

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

1

25

mcl

425美元

Immunoprecipitation of rat brain lysates: - 1. Cell lysates.2. Cell lysates + protein A beads + Anti-NMDAR2B (GluN2B) (extracellular) Antibody (#AGC-003). 3. Cell lysates + protein A beads + pre-immune rabbit serum.  Red arrow indicates the NMDA receptor 2B protein while the black arrow shows the IgG heavy chain. Immunoblot was performed with Anti-NMDAR2B (GluN2B) (extracellular) Antibody.