产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗GluR2(GluA2)(细胞外)抗体
目录 :
AGC-005
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-自由浮动切片
更多信息或购买 :
文章摘录数: 9
出版应用/物种/样本/稀释参考文献
  • 免疫组化-自由浮动切片; 小鼠; 1:500; 图 4a
Yeung J, Palpagama T, Tate W, Peppercorn K, Waldvogel H, Faull R, et al. The Acute Effects of Amyloid-Beta1-42 on Glutamatergic Receptor and Transporter Expression in the Mouse Hippocampus. Front Neurosci. 2019;13:1427 pubmed 出版商
  • 免疫印迹; 大鼠; 1:75; 图 2a
Egbenya D, Hussain S, Lai Y, Xia J, Anderson A, Davanger S. Changes in synaptic AMPA receptor concentration and composition in chronic temporal lobe epilepsy. Mol Cell Neurosci. 2018;92:93-103 pubmed 出版商
Metzdorf K, Fricke S, Balia M, Korte M, Zagrebelsky M. Nogo-A Modulates the Synaptic Excitation of Hippocampal Neurons in a Ca2+-Dependent Manner. Cells. 2021;10: pubmed 出版商
Li L, Chen S, Zhou M, Wang L, Li D, Chen H, et al. α2δ-1 switches the phenotype of synaptic AMPA receptors by physically disrupting heteromeric subunit assembly. Cell Rep. 2021;36:109396 pubmed 出版商
Helm M, Dankovich T, Mandad S, Rammner B, Jähne S, Salimi V, et al. A large-scale nanoscopy and biochemistry analysis of postsynaptic dendritic spines. Nat Neurosci. 2021;24:1151-1162 pubmed 出版商
Elhussiny M, Carini G, Mingardi J, Tornese P, Sala N, Bono F, et al. Modulation by chronic stress and ketamine of ionotropic AMPA/NMDA and metabotropic glutamate receptors in the rat hippocampus. Prog Neuropsychopharmacol Biol Psychiatry. 2021;104:110033 pubmed 出版商
Salpietro V, Dixon C, Guo H, Bello O, Vandrovcova J, Efthymiou S, et al. AMPA receptor GluA2 subunit defects are a cause of neurodevelopmental disorders. Nat Commun. 2019;10:3094 pubmed 出版商
Haglerød C, Hussain S, Nakamura Y, Xia J, Haug F, Ottersen O, et al. Presynaptic PICK1 facilitates trafficking of AMPA-receptors between active zone and synaptic vesicle pool. Neuroscience. 2017;344:102-112 pubmed 出版商
Szczurowska E, Ergang P, Kubová H, Druga R, Salaj M, MareÅ¡ P. Influence of early life status epilepticus on the developmental expression profile of the GluA2 subunit of AMPA receptors. Exp Neurol. 2016;283:97-109 pubmed 出版商
图像
图像 1 :
Alomone Labs AGC-005 图像 1
Western blot analysis of rat brain membranes: - 1. Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005), (1:200).2. Anti-GluR2 (GluA2) (extracellular) Antibody, preincubated with GluR2/GluA2 (extracellular) Blocking Peptide (#BLP-GC005).
图像 2 :
Alomone Labs AGC-005 图像 2
Expression of AMPA Receptor 2 (GluA2) in rat cerebellum - Immnunohistochemical staining of rat cerebellum using Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005) . A. Co-localization of GluA2 (green) and calbindin D28-K (red) in Purkinje cell dendrites (vertical arrow). B. Distribution of calbindin D28-K. C. Distribution of GluA2. Note that some GluA2 positive fibers (horizontal arrow) that are probable Bergmann glial processes do not stain for GluA2. D. The molecular and Purkinje layer (blue).
图像 3 :
Alomone Labs AGC-005 图像 3
Expression of GFAP in rat brain - Immunohistochemical staining of free-floating rat frozen brain sections using Anti-GFAP Antibody (#AFP-001) (1:2000). In rat parietal cortex GFAP immunoreactivity (green) appears in astrocytes (arrows). Nuclei are stained using DAPI as the counterstain (blue).
产品信息
SKU号 :
AGC-005_1_25_mcl
ParentSKU :
AGC-005
Taxonomy Node :
Primary Antibody>Extracellular>Non-Labeled
Action :
Insert
产品名称 :
抗GluR2(GluA2)(细胞外)抗体
产品描述 :
Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005) is a highly specific antibody directed against an epitope of the rat ionotropic glutamate receptor 2. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and immunoprecipitation applications. It has been designed to recognize GluR2 from human, mouse, and rat samples.
Short Description :
A Rabbit Polyclonal Antibody to AMPA-Selective Glutamate Receptor 2 (GluR2)
Negative Control :
BLP-GC005
同义词 :
GluR2 (GluA2), AMPA receptor 2, Glutamate receptor 2, Ionotropic glutamate receptor 2, AMPA-selective glutamate receptor 2, GRIA2, GluR-B, GluR-K2
靶标名 :
AMPA-Selective Glutamate Receptor 2 (GluR2)
Primary Category (Old) :
Ion Channels > Ligand-Gated Ion Channels > Glutamate Receptors > AMPA Receptors > Antibodies to AMPA Receptors
标记/共轭标签 :
未共轭
应用 :
免疫细胞化学, IF, 免疫组化, 免疫沉淀, LCI, 免疫印迹
宿主 :
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
GRIA2
反应物种 :
Mouse (Tested);Rat (Tested);Human (Predicted)
同源 :
Mouse - identical; human - 14/15 amino acid residues identical.
靶标/背景信息 :
AMPA receptors are members of the glutamate receptor family of ion channels that also include the NMDA and Kainate receptors. The three subfamilies are named after the original synthetic agonists that were identified as selective ligands of each family. The α-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) receptor subfamily includes four members AMPA1-AMPA4 that are also known as GluR1-GluR4 respectively. The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being actually heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3, although homotetramers can also be found. AMPA receptors are permeable to cations Na+, K+ and Ca2+. The Ca2+ permeability is dependent on the presence of AMPA2. The Ca2+ permeability of AMPA2 subunit is determined by the presence of the amino acid arginine (R) at a critical site in the pore loop instead of glutamine (Q) present in the same site in the other AMPA subunits. A post-transcriptional process known as RNA editing determines the presence of this R. Since most AMPA2 subunits in the adult brain have undergone RNA editing and most AMPA receptors contain the AMPA2 subunit, most native AMPA receptors will be impermeable to Ca2+. Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane. Synaptic strength, defined as the level of post-synaptic depolarization, can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning. Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has been focused on understanding the mechanisms that govern AMPA receptor trafficking.
免疫原 :
合成肽
区域 :
细胞外
Loop :
细胞外, N末端
序列 :
NVGNINNDKKDETYR(C), corresponding to amino acid residues 179-193 of rat GluR2
序列文本 :
Peptide NVGNINNDKKDETYR(C), corresponding to amino acid residues 179-193 of rat GluR2 (Accession P19491). Extracellular, N-terminus.
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.6毫克/毫升
浓度 :
06毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P19491
Top Related :
AGC-004, AGC-010, AGP-046, AGC-001, AGC-002
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
IP Legend :
Immunoprecipitation of rat brain lysates: - 1. Brain lysates + protein A beads + Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005).2. Brain lysates + protein A beads + pre-immune rabbit serum.3. Brain lysates.Black arrow indicates the GluR2 protein while the red arrow shows the IgG heavy chain. The immunoblot was performed with Anti-GluR2 (GluA2) (extracellular) Antibody (#AGC-005).
更多信息或购买 :
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年,在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。