产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗GluR3(GluA3)(细胞外)抗体
目录 :
AGC-010
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫印迹基因敲除验证
更多信息或购买 :
文章摘录数: 6
出版应用/物种/样本/稀释参考文献
  • 免疫印迹基因敲除验证; 小鼠; 1:100; 图 1d
García Hernández S, Abe M, Sakimura K, Rubio M. Impaired auditory processing and altered structure of the endbulb of Held synapse in mice lacking the GluA3 subunit of AMPA receptors. Hear Res. 2017;344:284-294 pubmed 出版商
Niu F, Zhang B, Feng J, Mao X, Xu X, Dong J, et al. Protein profiling identified mitochondrial dysfunction and synaptic abnormalities after dexamethasone intervention in rats with traumatic brain injury. Neural Regen Res. 2021;16:2438-2445 pubmed 出版商
Peter S, Urbanus B, Klaassen R, Wu B, Boele H, Azizi S, et al. AMPAR Auxiliary Protein SHISA6 Facilitates Purkinje Cell Synaptic Excitability and Procedural Memory Formation. Cell Rep. 2020;31:107515 pubmed 出版商
Jiang S, Lin Y, Hsieh C. Electroacupuncture at Hua Tuo Jia Ji Acupoints Reduced Neuropathic Pain and Increased GABAA Receptors in Rat Spinal Cord. Evid Based Complement Alternat Med. 2018;2018:8041820 pubmed 出版商
Parkinson G, Chamberlain S, Jaafari N, Turvey M, Mellor J, Hanley J. Cortactin regulates endo-lysosomal sorting of AMPARs via direct interaction with GluA2 subunit. Sci Rep. 2018;8:4155 pubmed 出版商
Koszegi Z, Fiuza M, Hanley J. Endocytosis and lysosomal degradation of GluA2/3 AMPARs in response to oxygen/glucose deprivation in hippocampal but not cortical neurons. Sci Rep. 2017;7:12318 pubmed 出版商
图像
图像 1 :
Alomone Labs AGC-010 图像 1
Western blot analysis of rat cerebellum lysates: - 1. Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010), (1:400).2. Anti-GluR3 (GluA3) (extracellular) Antibody, preincubated with GluR3/GluA3 (extracellular) Blocking Peptide (#BLP-GC010).
图像 2 :
Alomone Labs AGC-010 图像 2
Expression of GRIA3 (GluR3) in mouse cerebellum - Immunohistochemical staining of frozen perfusion-fixed free floating sections of mouse cerebellum using Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010). A. Distribution of GRIA3 (red). B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that GRIA3 is localized to Bergmann glia (vertical arrow) and to Purkinje cell soma (horizontal arrow). DAPI is used as the counterstain (blue).
产品信息
SKU号 :
AGC-010_1_25_mcl
ParentSKU :
AGC-010
Taxonomy Node :
Primary Antibody>Extracellular>Non-Labeled
Action :
Insert
产品名称 :
抗GluR3(GluA3)(细胞外)抗体
产品描述 :
Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010) is a highly specific antibody directed against an epitope of the rat ionotropic glutamate receptor 3. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, live cell imaging, and immunoprecipitation applications. It has been designed to recognize GluR3 from rat, mouse, and human samples.
Short Description :
A Rabbit Polyclonal Antibody to AMPA-Selective Glutamate Receptor 3 (GluR3)
Negative Control :
BLP-GC010
同义词 :
GluR3 (GluA3), AMPA Receptor 3, Glutamate receptor 3, Ionotropic glutamate receptor 3, AMPA-selective glutamate receptor 3, GRIA3, GluR-C, GluR-K3
靶标名 :
AMPA-Selective Glutamate Receptor 3 (GluR3)
Primary Category (Old) :
Ion Channels > Ligand-Gated Ion Channels > Glutamate Receptors > AMPA Receptors > Antibodies to AMPA Receptors
标记/共轭标签 :
未共轭
应用 :
免疫细胞化学, IF, 免疫组化, 免疫沉淀, LCI, 免疫印迹
宿主 :
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
GRIA3
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Mouse, human, dog - identical.
靶标/背景信息 :
L-Glutamate, the major excitatory neurotransmitter in the central nervous system, operates through several receptors that are categorized as ionotropic (ligand-gated cation channels) or metabotropic (G-protein coupled receptors). The ligand-gated ion channel family consists of 15 members that have been subdivided into three families based on their pharmacological profile: the a-amino-3-hydroxy-5-methyl-4-isoazolepropionic acid (AMPA) preferring receptors, the N-methyl-D-aspartate (NMDA) preferring and the kainate preferring receptors. The AMPA receptor subfamily includes four members AMPA1 to AMPA4, also known as GluR1 to GluR4 respectively. The functional AMPA channel is believed to be a tetramer, with most neuronal AMPA receptors being heterotetramers composed of AMPA1 plus AMPA2 or AMPA2 plus AMPA3 channels, although homotetramers can also been found. AMPA receptors are permeable to cations Na+, K+ and Ca2+. The Ca2+ permeability is dependent on the presence of AMPA2: whenever this subunit is present, the channel will be impermeable to Ca2+.1 Gating of AMPA receptors by glutamate is extremely fast and therefore the AMPA receptors mediate most excitatory (depolarizing) currents in the brain during basal neuronal activity. The depolarization caused by the activation of post-synaptic AMPA receptors is necessary for the activation of NMDA receptors that will open only in the presence of both glutamate and a depolarized membrane potential. Synaptic strength that is defined as the level of post-synaptic depolarization can be long term (hence the term long term potentiation, LTP) and therefore induce changes in signaling and protein synthesis in the activated neuron. These changes are associated with memory formation and learning. Changes in synaptic strength are thought to involve rapid movement of the AMPA receptors in and out of the synapses and a great deal of effort has focused in understanding the mechanisms that govern AMPA receptor trafficking.2 The exact physiological role of the AMPA3 receptor is not clear but a role in the modulation of oscillatory networks affecting sleep and breathing has been suggested.3
免疫原 :
合成肽
区域 :
细胞外
Loop :
细胞外, N末端
序列 :
(C)EKPFHLNYHVDHLD, corresponding to amino acid residues 60-73 of rat GluR3
序列文本 :
Peptide (C)EKPFHLNYHVDHLD, corresponding to amino acid residues 60-73 of rat GluR3 (Accession P19492). Extracellular, N-terminus.
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.6毫克/毫升
浓度 :
06毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P19492
Top Related :
AGC-019, AGC-005, AGC-004, AGP-009, APZ-010
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
IP Legend :
Immunoprecipitation of rat cerebellum lysates: - 1. Cerebellum lysates.2. Cerebellum lysates + Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010) + protein A  beads.3. Cerebellum lysates + pre-immune rabbit serum + protein A beads.Black arrow indicates GluR3 while the red arrow shows the IgG heavy chain. Immunoblot was performed with Anti-GluR3 (GluA3) (extracellular) Antibody (#AGC-010).
更多信息或购买 :
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年,在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。