抗体

抗GLP1R(细胞外)抗体

AGR-021

1 X 50 mcl

530美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

AGR-021

Primary Antibody>Extracellular>Non-Labeled

Insert

抗GLP1R(细胞外)抗体

Anti-GLP1R (extracellular) Antibody (#AGR-021) is a highly specific antibody directed against an epitope of the rat glucagon-like peptide 1 receptor. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell imaging applications. It has been designed to recognize GLP1R from human, rat, and mouse samples.

A兔多克隆抗体胰高血糖素样肽受体

BLP-GR021

GLP1R, 胰高血糖素样肽受体, GLP-1受体

胰高血糖素样肽受体

G-Protein Coupled Receptors > Glucagon Receptor Family > Antibodies to Glucagon Receptor Family

未共轭

免疫细胞化学, IF, IFC, 免疫组化, LCI, 免疫印迹

兔

多克隆

兔IgG

GLP1R

人类(试验);小鼠(试验);大鼠(试验)

Rat, mouse, human - 13/14 amino acid residues identical.

Glucagon-like peptide 1 (GLP-1) is a hormone secreted by the intestine following a meal1. This hormone is known to enhance the secretion of insulin, important glucose blood level regulation2,3. In pancreatic β-cells, other than stimulating insulin secretion, GLP-1 action is also important for β-cell mass expansion2. GLP-1 exerts its biological functions through Glucagon-like peptide 1 receptor (GLP-1 receptor), a member of the G-protein coupled receptor superfamily. Like all members, this receptor spans the membrane seven times. GLP-1 receptor couples with Gs, thereby stimulating adenylate cyclase, consequently leading to an increase in cAMP2. The receptor is expressed in various tissues, namely the pancreas, adipose tissue, muscle, heart, gastrointestinal tract and liver. GLP-1 receptor is also found in the central nervous system where it provides a neuroprotective effect4. The glucagon-like peptide 1 system may serve as a therapeutic treatment in diabetes mellitus, in order to maintain β-cell mass expansion2. In addition, the receptor may serve as a therapy target in various neurodegenerative diseases like Alzheimer’s and Parkinson’s disease4.

合成肽

细胞外

2nd extracellular loop

(C)KYLYEDEG(S)WTRNS, corresponding to amino acid residues 288-301 of rat GLP1R

Peptide (C)KYLYEDEG(S)WTRNS, corresponding to amino acid residues 288-301 of rat GLP1R (Accession P32301). 2nd extracellular loop.

1 X 50 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P32301

AGR-024, AGR-022, AGR-031, ANR-025, ANR-022

Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

50

mcl

530美元

Cell surface detection of Glucagon-like peptide 1 receptor by indirect flow cytometry in live intact mouse J774 macrophage cells:  Cell surface detection of Glucagon-like peptide 1 receptor by indirect flow cytometry in live intact mouse J774 macrophage cells:  Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells + Anti-GLP1R (extracellular) Antibody (#AGR-021), (2.5μg) + goat-anti-rabbit-FITC.

Expression of Glucagon-like peptide 1 receptor in rat pancreatic islet cells - Cell surface detection of Glucagon-like peptide 1 receptor in live intact rat RIN-5F pancreatic islet cells. A. Cells were stained with Anti-GLP1R (extracellular) Antibody (#AGR-021), (1:100), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Cell nuclei were visualized with the membrane-permeable DNA dye Hoechst 33342 (blue staining). C. Live view of the cells.