产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗神经生长因子抗体
目录 :
AN-240
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
文章摘录数: 12
| 参考文献 |
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图像
图像 1 :
Western blot analysis using Anti-NGF Antibody (#AN-240) (1:200).
图像 2 :
Expression of NGF in rat brain - Immunohistochemical staining of rat hippocampal mossy fiber terminal zone using Anti-NGF Antibody (#AN-240). A. The mossy fiber terminal zone (MF) is seen as the gray area (arrow) adjacent to the pyramidal layer (P). The pyramidal layer is visualized using cresyl violet (purple-labeled cells). B. The antibody was pre-incubated with the immunogen. The lack of staining in the MF zone (arrow) demonstrates specificity.
图像 3 :
Expression of NGF in rat brain - Immunohistochemical staining of rat hippocampal mossy fiber terminal zone usingAnti-NGFAntibody (#AN-240). A. The mossy fiber terminal zone (MF) is seen as the gray area (arrow) adjacent to the pyramidal layer (P). The pyramidal layer is visualized using cresyl violet (purple-labeled cells). B. The antibody was pre-incubated with the immunogen. The lack of staining in the MF zone (arrow) demonstrates specificity.
产品信息
SKU号 :
AN-240_1_25_mcl
ParentSKU :
AN-240
Taxonomy Node :
Primary Antibody>Intracellular>Non-Labeled
Action :
Insert
产品名称 :
抗神经生长因子抗体
产品描述 :
Alomone Labs is pleased to offer a highly specific antibody directed against mouse NGF. Anti-NGF Antibody (#AN-240) can be used in western blot and immunohistochemical applications. It has been designed to recognize NGF from mouse, rat and human samples. The antibody also recognizes proNGF.
nRelated products for neutralizing NGF activity:
nMouse NGF/proNGF Neutralizing Antibody (#ALM-006) can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.
Short Description :
A兔多克隆抗体神经生长因子
Negative Control :
BLP-N240
同义词 :
神经生长因子, 神经生长因子
靶标名 :
神经生长因子
Primary Category (Old) :
Neurotrophic Factors and Receptors > Nerve Growth Factor (NGF) > Antibodies
标记/共轭标签 :
未共轭
应用 :
IF, 免疫组化, 免疫印迹
宿主 :
兔
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
神经生长因子
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Rat- 115/120 amino acid residues identical; human- 107/120 amino acid residues identical.
靶标/背景信息 :
The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and the PNS.1 NGF, the first member of the family to be discovered, was originally purified as a factor able to support survival of sympathetic and sensory spinal neurons in culture.2 It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their final target.3 Neurotrophin secretion also increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however direct trauma and induced cytokines combine to increase neurotrophin production in these cells after injury.4
NGF is purified in three forms: the 7S, 2.5S and β. The 7S, 130 kDa, form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two α, one β and two γ subunits. The name is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the β, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino acids shorter than the β form, because of proteolysis that occurs during the purification process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.7 There is a 95.8% homology between the rat and mouse forms, and a 85% homology between the human and mouse.
NGF has been shown to regulate neuronal survival, development function and plasticity.8 Recently, involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,9 psoriasis10 and wound healing.11
The biological effects of NGF are mediated by two receptors:
TrkA, which is specific for NGF, and p75, which binds all the neurotrophins.12
免疫原 :
合成肽
区域 :
intracellular
Loop :
Intracellular
序列 :
Highly purified 2.5 S mouse NGF
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.3毫克/毫升
浓度 :
03毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose (no preservative).
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P01139
Top Related :
蚂蚁-005, 蚂蚁-010, 蚂蚁-006, 蚂蚁-014, N-100
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
更多信息或购买 :
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年，在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。