抗体

抗Lynx1(细胞外)抗体

ANC-021

1 X 50 mcl

530美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

ANC-021

Primary Antibody>Extracellular>Non-Labeled

Insert

抗Lynx1(细胞外)抗体

Anti-Lynx1 (extracellular) Antibody (#ANC-021) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, indirect live cell flow cytometry, and immunohistochemistry applications. It has been designed to recognize Lynx1 from rat, mouse, and human samples.

A兔多克隆抗体Lynx1

BLP-NC021

Ly-6/Neurotoxin-like protein 1, Endogenous prototoxin LYNX1

Lynx1

Ion Channels > Ligand-Gated Ion Channels > Nicotinic Acetylcholine Receptors > Antibodies to Interacting Proteins

未共轭

IF, IFC, 免疫组化, 免疫印迹

兔

多克隆

兔IgG

LYNX1

人类(试验);小鼠(试验);大鼠(试验)

Mouse, rat – 12/13 amino acid residues identical; human – 11/13 amino acid residues identical.

Allograft Inflammatory Factor-1 (AIF1) is a 17 kDa. cytoplasmic, calcium and actin binding scaffold protein, mainly expressed in immunocytes. AIF-1 is induced by cytokines such as interferon-γ (IFN-γ). It is encoded within the HLA class III genomic region by IBA1 gene, and its structure contains seven alpha helixes and three beta sheets. There are three other proteins identical to AIF1: Ionized Ca2+-Binding Adaptor 1, Microglia Response Factor-1, and Daintain1. The gene is highly expressed in testis, spleen, circulating macrophages and in the brain but weakly expressed in lung and kidney. Among brain cells, the Iba1 gene is strongly and specifically expressed in microglia and its expression is up-regulated following nerve injury, central nervous system ischemia, and several other brain diseases2. AIF1 influences the immune system at several key points and thus modulates inflammatory diseases. It increases the expression of inflammatory mediators such as cytokines, chemokines, inducible nitric oxide synthase (iNOS) and promotes inflammatory cell proliferation and migration and hence it is highly associated with autoimmune diseases3. In addition, its abundance is positively correlated with metabolic indicators, such as body mass index, triglycerides, and fasting plasma glucose levels. There is also evidence that AIF1 could be a marker for diabetic nephropathy when detected in serum. It is found in activated macrophages in the pancreatic islets, and has been shown to decrease insulin secretion, while simultaneously impairing glucose elimination4.

合成肽

细胞外

细胞外, N末端

CAYNGDN(S)FKPMR, corresponding to amino acid residues 26-38 of mouse Lynx1

Peptide CAYNGDN(S)FKPMR, corresponding to amino acid residues 26-38 of mouse Lynx1 (Accession P0DP60). Extracellular, N-terminus.

1 X 50 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P0DP60

Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

50

mcl

530美元

Cell surface detection of Lynx1 in live intact human THP-1 monocytic leukemia cells: Cell surface detection of Lynx1 in live intact human THP-1 monocytic leukemia cells: Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells + Anti-Lynx1 (extracellular) Antibody (#ANC-021), (2.5 µg) + goat-anti-rabbit-FITC.