抗体

抗SNAP-25抗体

ANR-001

1 X 50 mcl

530美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

ANR-001

Primary Antibody>Intracellular>Non-Labeled

Insert

抗SNAP-25抗体

Alomone Labs is pleased to offer a highly specific antibody directed against rat SNAP-25. Anti-SNAP-25 Antibody (#ANR-001) can be used in western blot and immunohistochemical applications. It has been designed to recognize SNAP-25 from rat, mouse and human samples.

A兔多克隆抗体SNAP-25

BLP-NR001

SNAP-25, Synaptosomal-associated protein 25 kD

SNAP-25

Neural Signaling > Exocytotic Machinery Proteins > Antibodies

未共轭

IF, 免疫组化, 免疫印迹

兔

多克隆

兔IgG

SNAP25

人类(试验);小鼠(试验);大鼠(试验)

Human, mouse - identical.

SNAP-25 (synaptosomal-associated 25kD protein) is a member of the soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) protein superfamily. The family includes 36 members in humans and is characterized by the SNARE motif, an evolutionarily conserved stretch of 60–70 amino acids that are arranged in heptad repeats1,2. SNARE proteins are involved in exocytosis and intracellular vesicle trafficking and are essential for cell growth, hormone secretion and neurotransmission, processes that require rapid, targeted, and regulated membrane fusion1,2. SNAREs can be roughly divided into vesicular (v-SNAREs) and target (t-SNAREs) based on their distribution on the transport vesicle or target membrane respectively. Thus, assembly of cognate v-/t-SNAREs between two opposing membranes generates trans-SNARE complexes, which bring the lipid bilayers in close proximity and drive membrane fusion. SNAP-25, unlike most SNAREs, lacks a membrane-spanning region, but is attached to the cytoplasmic side of the plasma membrane by posttranslational modifications such as palmitoylation or farnesylation1,2. SNAP-25 has been extensively studied for its role on neuronal and neuroendocrine cell exocytosis where it functions as one of the plasma membrane protein t-SNARE, which together with the vesicular v-SNARE protein VAMP and another t-SNARE such as Syntaxin 1, forms a trimeric, four-helical complex, which drives fusion of the two opposing bilayers1,2. SNAP-25 is the target of several botulinum neurotoxin (BoNT) types: type A, C and E. The neurotoxins cause specific proteolytic degradation of the SNAP-25 protein, which in turn causes SNARE complex disruption and inhibition of neurotransmitter release3.

谷胱甘肽S-转移酶融合蛋白

intracellular

Cytoplasmic

VDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG, corresponding to the full-length of rat SNAP-25
MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEES
KDAGIRTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNL
TDLGKFCGLCVCPCNKLKSSDAYKKAWGNNQDGVVASQP
ARV
GST fusion protein with the sequence

1 X 50 mcl

1毫克/毫升

1毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen

-20°C

P60881

ASC-002

Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

50

mcl

530美元