抗体

抗突触素抗体

ANR-013

1 X 50 mcl

530美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

ANR-013

Primary Antibody>Intracellular>Non-Labeled

Insert

抗突触素抗体

Anti-Synaptophysin Antibody (#ANR-013) is a highly specific antibody directed against an epitope of the rat protein. It can be used in western blot and immunohistochemistry applications. It has been designed to recognize synaptophysin from mouse, rat, and human samples.

A兔多克隆抗体突触素

BLP-NR013

Synaptophysin, Major synaptic vesicle protein p38, SYP

突触素

Neural Signaling > Exocytotic Machinery Proteins > Antibodies

未共轭

IF, 免疫组化, 免疫印迹

兔

多克隆

兔IgG

SYP

Mouse (Tested);Rat (Tested);Human (Predicted)

Rat, mouse, human - 12/13 amino acid residues identical.

Synaptophysin is the major integral membrane protein of small synaptic vesicles1-3. It belongs to a family of proteins which includes synaptogyrin and synaptoporin. The protein contains 4 transmembrane domains and cytoplasmic N- and C-termini4. There are two intravesicular loops that contain disulfide bonds. The protein is N-glycosylated on the first intravesicular loop, a modification important for the synaptic vesicle targeting of synaptophysin. Indeed, a mutant form of the protein, unable to undergo glycolysation, leads to the accumulation of the protein in the cell body of neurons indicating that N-glycosylation is required for the synaptic localization of synaptophysin5. The C-terminal tail is extensively phosphorylated on Tyr residues. However, the biological significance of the post-translational modification is not yet determined6. Although exact and specific role of synaptophysin remains a mystery, a study suggests that the protein is required for efficient endocytosis of synaptic vesicles in cultured hippocampal neurons6. Since synaptophysin is exclusively detected in synaptic vesicles, it is widely used as a marker for presynaptic vesicles. Genetic screening in humans and behavioral studies in rodents show that loss of synaptophysin expression or expression of a truncated form of the protein may be attributed to mental retardation and/or learning deficits7,8. Interestingly though, synaptic transmission in synaptophysin knockout mice remains normal6.

合成肽

intracellular

2nd intravesicular loop

CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat synaptophysin

Peptide CRQTGNT(S)KELRD, corresponding to amino acid residues 178-190 of rat synaptophysin (Accession P07825). 2nd intravesicular loop.

1 X 50 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P07825

ANR-014, APZ-009, ANR-003, ANR-113, ANR-111

Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

50

mcl

530美元