抗体

抗CD56/神经细胞粘附分子1(细胞外)抗体

ANR-041

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学, 免疫印迹基因敲除验证

ANR-041

Primary Antibody>Extracellular>Non-Labeled

Insert

抗CD56/神经细胞粘附分子1(细胞外)抗体

Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041) is a highly specific antibody directed against rat Neural cell adhesion molecule 1. The antibody can be used in western blot, live cell imaging, and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize NCAM1 from rat, mouse and human samples.

A兔多克隆抗体神经细胞粘附分子1(CD56)

BLP-NR041

CD56(神经细胞粘附分子1), 神经细胞粘附分子1, NCAM-1

神经细胞粘附分子1(CD56)

Neural Signaling > Neuronal Adhesion Molecules > Antibodies

未共轭

免疫细胞化学, IF, IFC, 免疫组化, LCI, 免疫印迹

兔

多克隆

兔IgG

神经细胞粘附分子1

人类(试验);小鼠(试验);大鼠(试验)

Mouse - identical; human - 13/15 amino acid residues identical.

Neural cell adhesion molecule 1 (NCAM-1) is a member of a large family of cell-surface glycoproteins and plays a major role during development and controls various functions in the nervous system such as cell migration, neurite growth, axonal guidance, and synaptic plasticity1. NCAM-1 mediates adhesion between cells through homophilic bonds formed between its extracellular domains, which comprise five tandem Ig domains and two juxtamembrane fibronectin type III (Fn III) domains2. There are three major protein isoforms of NCAM-1: 180 kd, 140 kd, and 120 kd. The 180-kd and 140-kd isoforms of NCAM-1 are transmembrane proteins, whereas the 120-kd isoform is linked to the plasma membrane via a glycosyl phosphatidyl-inositol (GPI) lipid anchor3.  NCAM-1 is widely expressed in the central nervous system, skeletal muscle and natural killer cells4. Several lines of evidence have supported the idea that dysregulation of NCAM-1 isoforms in the brain might be involved in the pathophysiology of neuropsychiatric disorders, particularly bipolar affective disorder5.

合成肽

细胞外

细胞外, N末端

(C)DEKHIFSDDSSELTIR, corresponding to amino acid residues 261-276 of rat NCAM-1

Peptide (C)DEKHIFSDDSSELTIR, corresponding to amino acid residues 261-276 of rat NCAM-1 (Accession P13596). Extracellular, N-terminus.

1 X 25 mcl

0.85毫克/毫升

085毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P13596

ASC-009, ANR-031-AG, 蚂蚁-014, AGC-021

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元

Cell surface detection of NCAM1 by indirect flow cytometry in live intact human natural killer cells NK-92MI cells: Cell surface detection of NCAM1 by indirect flow cytometry in live intact human natural killer cells NK-92MI cells: Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells + Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041), (5μg) + goat-anti-rabbit-FITC.

Expression of NCAM1 in rat PC12 cells - Cell surface detection of NCAM1 in intact living rat pheochromocytoma (PC12) cells using Anti-CD56/NCAM1 (extracellular) Antibody (#ANR-041), (1:100). A, B. Extracellular staining of cells merged with a live view of the cells.