抗体

抗钙粘蛋白N抗体

ANR-082

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化

ANR-082

Primary Antibody>Intracellular>Non-Labeled

Insert

抗钙粘蛋白N抗体

Anti-N-Cadherin Antibody (#ANR-082) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize CDH2 from rat, mouse, and human samples.

A兔多克隆抗体钙粘蛋白N

BLP-NR082

N-Cadherin, Cadherin 2, Neural cadherin, CDH2, NCAD, CDHN, CD325, Calcium-dependent adhesion protein neuronal

钙粘蛋白N

Pharmacology Targets > Other Protein Targets > Adhesion Molecules

未共轭

免疫组化, 免疫印迹

兔

多克隆

兔IgG

CDH2

人类(试验);小鼠(试验);大鼠(试验)

Rat, human – identical.

Cadherins are transmembrane proteins responsible for cell adhesion. Cell adhesion is a crucial feature for the sustainability and maintenance of normal tissue function and three dimensional structure. Cadherins share an extracellular domain consisting of multiple repeats of cadherin-specific motif and are calcium dependent homotypic cell-to-cell adhesion molecules. They are localized mostly in specialized sites called adherence junctions1. N-cadherin consists of an amino-terminal external domain with five tandem repeats, a single transmembrane segment, and a cytoplasmic carboxy-terminal domain of approximately 150 amino acids. The intracellular domain of N-cadherin interacts with a group of proteins called catenins that are essential for cadherin-mediated cell adhesion. It is suggested that N-cadherin proteins align in a form of “zipper” when involved in cell adhesion. Cadherins on one cell surface form a series of rigid dimers that attach to equivalent dimers on the opposing cells and lateral motion of these complexes allows the cell junction site to “zip up”1. N-cadherin plays a role in tumor metastasis. Unlike E-cadherin that causes tumor metastasis when insufficiently, N-cadherin is involved in tumor metastasis when upregulated. N-cadherin stimulates migration, invasion and metastasis of squamous cell breast cancer and its effects are increased by FGF-2 suggesting that N-cadherin and FGFR-1 synergize and promote aggressive tumor behavior. This synergy also promotes neuronal growth1. Interestingly, low levels of N-cadherin are associated with a higher invasive capacity of astrocytic glioma by increasing tumor (and normal) cell migration speed and creating a less organized pattern of migration3.

合成肽

intracellular

Intracellular

(C)DTVEPDAIKPVGIR, corresponding to amino acid residues 794-807 of mouse CDH2

Peptide (C)DTVEPDAIKPVGIR, corresponding to amino acid residues 794-807 of mouse CDH2 (Accession P15116). Intracellular.

1 X 25 mcl

0.85毫克/毫升

085毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P15116

AAR-016

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元