抗体

抗LRRK2抗体

ANR-102

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

ANR-102

Primary Antibody>Intracellular>Non-Labeled

Insert

抗LRRK2抗体

Anti-LRRK2 Antibody (#ANR-102) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize LRKK2 from human, mouse, and rat samples.

A兔多克隆抗体LRRK2

BLP-NR102

LRRK2, Leucine-rich repeat kinase 2, Parkinson disease (Autosomal dominant) 8, PARK8, Roco2, RIPK7, Dardarin

LRRK2

Neural Signaling > Leucine-Rich Repeat Kinase (LRRK) Family > Antibodies

未共轭

IF, 免疫组化, 免疫印迹

兔

多克隆

兔IgG

LRRK2

人类(试验);小鼠(试验);大鼠(试验)

Rat, human – identical.

Parkinson’s disease (PD) is the second most common neurodegenerative disease. The condition is inherited in 5-10% of all PD patients. Mutations in the PARK8 gene have been implicated in the development of PD. PARK8 gene encodes the protein LRRK2 (Leucine-rich repeat kinase 2). It is a multi-domain protein containing distinctive enzymatic domains of GTPase and a kinase, the protein interaction motifs LRR (Leucine-rich repeat) and WD40. LRRK2’s domain structure indicates that it belongs to the Roco protein family. LRRK2 is expressed in the kidney, lung and dopaminergic neurons in the substantia nigra. It is mostly located in the cytosol, however, it is also expressed on membranes of organelles such as the mitochondria, golgi apparatus and synaptic vesicles. There are several PARK8 mutations that have been associated with both familial and sporadic PD. The most common mutation, G2019S, is present in 85% of PD patients carrying PARK8 mutations and in 4% of all familial PD patients. In addition, this mutation has been identified in 1% of sporadic PD patients. The prevalence of this mutation is especially high in Ashkenazi Jewish and North-African Arab populations. This mutation causes increased kinase activity while other mutations, such as R1441C, decrease GTPase activity. Overexpression of the LRRK2 protein causes neuronal cell death and other pathological conditions which are aggravated by the overexpression of mutant proteins. The exact role of LRRK2 in PD pathophysiology still remains unclear1.

合成肽

intracellular

N末端

CKALHVLFERVSEE, corresponding to amino acid residues 183-196 of mouse LRRK2

Peptide CKALHVLFERVSEE, corresponding to amino acid residues 183-196 of mouse LRRK2 (Accession Q5S006). N-terminus.

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

Q5S006

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元