产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗proNGF抗体
目录 :
蚂蚁-005
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
文章摘录数: 10
参考文献
Marsland M, Dowdell A, Jiang C, Wilmott J, Scolyer R, Zhang X, et al. Expression of NGF/proNGF and Their Receptors TrkA, p75NTR and Sortilin in Melanoma. Int J Mol Sci. 2022;23: pubmed 出版商
Du Z, Song Y, Chen X, Zhang W, Zhang G, Li H, et al. Knockdown of astrocytic Grin2a aggravates β-amyloid-induced memory and cognitive deficits through regulating nerve growth factor. Aging Cell. 2021;20:e13437 pubmed 出版商
Li A, Feng L, Niu X, Zeng Q, Li B, You Z. Downregulation of OIP5-AS1 affects proNGF-induced pancreatic cancer metastasis by inhibiting p75NTR levels. Aging (Albany NY). 2021;13:10688-10702 pubmed 出版商
Pentz R, Iulita M, Mikutra Cencora M, Ducatenzeiler A, Bennett D, Cuello A. A new role for matrix metalloproteinase-3 in the NGF metabolic pathway: Proteolysis of mature NGF and sex-specific differences in the continuum of Alzheimer's pathology. Neurobiol Dis. 2021;148:105150 pubmed 出版商
Pham D, Bruelle C, Thi Do H, Pajanoja C, Jin C, Srinivasan V, et al. Caspase-2 and p75 neurotrophin receptor (p75NTR) are involved in the regulation of SREBP and lipid genes in hepatocyte cells. Cell Death Dis. 2019;10:537 pubmed 出版商
Elshaer S, El Remessy A. Deletion of p75NTR prevents vaso-obliteration and retinal neovascularization via activation of Trk- A receptor in ischemic retinopathy model. Sci Rep. 2018;8:12490 pubmed 出版商
Ryu J, Tooke K, Malley S, Soulas A, Weiss T, Ganesh N, et al. Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. 2018;128:1772-1786 pubmed 出版商
Mohamed R, Coucha M, Elshaer S, Artham S, Lemtalsi T, El Remessy A. Inducible overexpression of endothelial proNGF as a mouse model to study microvascular dysfunction. Biochim Biophys Acta Mol Basis Dis. 2018;1864:746-757 pubmed 出版商
Kim M, Vargas M, Harlan B, Killoy K, Ball L, Comte Walters S, et al. Nitration and Glycation Turn Mature NGF into a Toxic Factor for Motor Neurons: A Role for p75NTR and RAGE Signaling in ALS. Antioxid Redox Signal. 2018;28:1587-1602 pubmed 出版商
Mesentier Louro L, De Nicoló S, Rosso P, De Vitis L, Castoldi V, Leocani L, et al. Time-Dependent Nerve Growth Factor Signaling Changes in the Rat Retina During Optic Nerve Crush-Induced Degeneration of Retinal Ganglion Cells. Int J Mol Sci. 2017;18: pubmed 出版商
图像
图像 1 :
Alomone Labs ANT-005 图像 1
Western blot analysis of Recombinant mouse proNGF protein (#N-250), (lanes 1-3) and Recombinant human proNGF protein (#N-280), (lanes 4-6): - 1,4. 100 ng + Anti-proNGF Antibody (ANT-005), (1:200).2,5. 250 ng + Anti-proNGF Antibody (1:200).3,6. 500 ng + Anti-proNGF Antibody (1:200).
图像 2 :
Alomone Labs ANT-005 图像 2
Expression of proNGF in rat brain sections - Immunohistochemical staining of proNGF in rat brain sections using Anti-proNGF Antibody (#ANT-005). A. Staining of astrocytes was performed using mouse anti-glial fibrillary acidic protein (GFAP) (red). B. Co-localization between GFAP and proNGF (orange). C. Staining of proNGF (green).
图像 3 :
Alomone Labs ANT-005 图像 3
Western blot analysisof mouse submandibular glands (SMGs): - 1.Anti-proNGF Antibody (#ANT-005)(1:200).2.Anti-proNGF Antibody preincubated with the control peptide antigen.
产品信息
SKU号 :
蚂蚁-005_1_25_mcl
ParentSKU :
蚂蚁-005
Taxonomy Node :
Primary Antibody>Intracellular>Non-Labeled
Action :
Insert
产品名称 :
抗proNGF抗体
产品描述 :
Alomone Labs is pleased to offer a highly specific antibody directed against rat proNGF. Anti-proNGF Antibody (#ANT-005) can be used in western blot and immunohistochemical applications. The antibody specifically reacts with proNGF. It has been designed to recognize proNGF from mouse, rat and human samples. nRelated products for neutralizing proNGF activity: nMouse NGF/proNGF Neutralizing Antibody (#ALM-006) can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF.
Short Description :
A兔多克隆抗体proNGF
Negative Control :
BLP-NT005
同义词 :
proNGF, Nerve growth factor precursor
靶标名 :
proNGF
Primary Category (Old) :
Neurotrophic Factors and Receptors > Nerve Growth Factor (NGF) > Antibodies
标记/共轭标签 :
未共轭
应用 :
IF, 免疫组化, 免疫印迹
宿主 :
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
神经生长因子
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Mouse - identical; human - 16/21 amino acid residues identical.
靶标/背景信息 :
Neurotrophins are synthesized as pro-forms that can be cleaved either intracellularly to release mature, secreted ligands, or extracellularly by various proteases such as plasmin, furin, PC1/3, PC7, and PACE 4.1,2,5 The immature precursor has a prodomain of 103 amino acids, which was thought to have a role in the folding and sorting of the mature NGF into the various secretion pathways.  It was recently reported that proNGF, binds p75NTR receptor preferentially over TrkA, and this selective binding of proNGF to p75NTR leads to apoptotic death of cells that express both TrkA and p75NTR. However, mature NGF binds and activates both receptors, with resulting promotion of cell survival due to the TrkA-mediated survival signal overriding p75NTR -mediated apoptotic signal.3,4  Since pro- and mature neurotrophins seem to elicit opposite functional effects, by differential interactions with Trks and p75NTR receptors, extracellular cleavage represents a new way to control the synaptic functions of neurotrophins. It was demonstrated that proNGF from injured spinal cord extracts, is active and induce apoptosis among oligodendrocytes, and apoptosis can be blocked by a proNGF-specific antibody.6 Finally, proNGF was demonstrated as the predominant form in mouse, rat, and human brain tissue, thyroid, hippocampus, thus suggesting a role for proNGF in vivo.5,7,8
免疫原 :
合成肽
区域 :
intracellular
Loop :
Pro-domain of the NGF protein
序列 :
(C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor)
序列文本 :
Peptide (C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor) (Accession P25427). Pro-domain of the NGF protein.
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.8毫克/毫升
浓度 :
08毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P25427
Top Related :
AN-240, N-285, 蚂蚁-007, 蚂蚁-006, N-245
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
更多信息或购买 :
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年,在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。