产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗proBDNF抗体
目录 :
蚂蚁-006
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
文章摘录数: 13
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠; 1:400; 图 4a
Lin R, Chen J, Li X, Mao J, Wu Y, Zhuo P, et al. Electroacupuncture at the Baihui acupoint alleviates cognitive impairment and exerts neuroprotective effects by modulating the expression and processing of brain-derived neurotrophic factor in APP/PS1 transgenic mice. Mol Med Rep. 2016;13:1611-7 pubmed 出版商
Hossain M, Mawatari S, Fujino T. Plasmalogens, the Vinyl Ether-Linked Glycerophospholipids, Enhance Learning and Memory by Regulating Brain-Derived Neurotrophic Factor. Front Cell Dev Biol. 2022;10:828282 pubmed 出版商
Vignoli B, Sansevero G, Sasi M, Rimondini R, Blum R, Bonaldo V, et al. Astrocytic microdomains from mouse cortex gain molecular control over long-term information storage and memory retention. Commun Biol. 2021;4:1152 pubmed 出版商
Dong Y, Hong W, Tang Z, Gao Y, Wu X, Liu H. Dexmedetomidine Attenuates Neurotoxicity in Developing Rats Induced by Sevoflurane through Upregulating BDNF-TrkB-CREB and Downregulating ProBDNF-P75NRT-RhoA Signaling Pathway. Mediators Inflamm. 2020;2020:5458061 pubmed 出版商
Andreska T, Rauskolb S, Schukraft N, Lüningschrör P, Sasi M, Signoret Genest J, et al. Induction of BDNF Expression in Layer II/III and Layer V Neurons of the Motor Cortex Is Essential for Motor Learning. J Neurosci. 2020;40:6289-6308 pubmed 出版商
Montroull L, Rothbard D, Kanal H, D Mello V, Dodson V, Troy C, et al. Proneurotrophins Induce Apoptotic Neuronal Death After Controlled Cortical Impact Injury in Adult Mice. ASN Neuro. 2020;12:1759091420930865 pubmed 出版商
Hong J, Heo W. Optogenetic Modulation of TrkB Signaling in the Mouse Brain. J Mol Biol. 2020;432:815-827 pubmed 出版商
Montroull L, Danelon V, Cragnolini A, Mascó D. Loss of TrkB Signaling Due to Status Epilepticus Induces a proBDNF-Dependent Cell Death. Front Cell Neurosci. 2019;13:4 pubmed 出版商
Dong C, Tian Z, Zhang K, Chang L, Qu Y, Pu Y, et al. Increased BDNF-TrkB signaling in the nucleus accumbens plays a role in the risk for psychosis after cannabis exposure during adolescence. Pharmacol Biochem Behav. 2019;177:61-68 pubmed 出版商
Robinson A, Abraham C, Rosene D. Candidate molecular pathways of white matter vulnerability in the brain of normal aging rhesus monkeys. GeroScience. 2018;40:31-47 pubmed 出版商
Oliver R, Brigman J, Bolognani F, Allan A, Neisewander J, Perrone Bizzozero N. Neuronal RNA-binding protein HuD regulates addiction-related gene expression and behavior. Genes Brain Behav. 2018;17:e12454 pubmed 出版商
Danelon V, Montroull L, Unsain N, Barker P, Mascó D. Calpain-dependent truncated form of TrkB-FL increases in neurodegenerative processes. Mol Cell Neurosci. 2016;75:81-92 pubmed 出版商
de la Cruz Morcillo M, Berger J, Sanchez Prieto R, Saada S, Naves T, Guillaudeau A, et al. p75 neurotrophin receptor and pro-BDNF promote cell survival and migration in clear cell renal cell carcinoma. Oncotarget. 2016;7:34480-97 pubmed 出版商
图像
图像 1 :
Alomone Labs ANT-006 图像 1
Western blot analysis of rat glioma C6 (1, 3) or human neuroblastoma SH-SY5Y (2, 4) cell lysate: - 1,2. Anti-proBDNF Antibody (#ANT-006), (1:200).3,4. Anti-proBDNF Antibody, preincubated with proBDNF Blocking Peptide (#BLP-NT006).
图像 2 :
Alomone Labs ANT-006 图像 2
Expression of proBDNF in rat cerebellum - Immunohistochemical staining of rat cerebellum with Anti-proBDNF Antibody (#ANT-006). proBDNF (red) appears in Purkinje cell bodies (vertical arrows) and in astrocytic processes (horizontal arrows in A) but not in Purkinje neuronal dendrites stained for calbindin D28k (green, in B) in the same brain section. C. Confocal merge of proBDNF and CBD28K demonstrates the restriction of proBDNF to Purkinje cell body.
产品信息
SKU号 :
蚂蚁-006_1_25_mcl
ParentSKU :
蚂蚁-006
Taxonomy Node :
Primary Antibody>Intracellular>Non-Labeled
Action :
Insert
产品名称 :
抗proBDNF抗体
产品描述 :
Anti-proBDNF Antibody (#ANT-006) is a highly specific antibody directed against the prodomain region of human proBDNF. The antibody can be used in western blot, immunoprecipitation, and immunohistochemistry applications. It has been designed to recognize proBDNF from human, mouse, and rat samples. The antibody does not cross react with mature BDNF, pro- and mature NGF or mature NT-3.
Short Description :
A兔多克隆抗体proBDNF
Negative Control :
BLP-NT006
Positive Control :
PCP-B257
同义词 :
proBDNF, Brain-derived neurotrophic factor precursor
靶标名 :
proBDNF
Primary Category (Old) :
Neurotrophic Factors and Receptors > Neurotrophins > Antibodies
标记/共轭标签 :
未共轭
应用 :
IF, 免疫组化, 免疫沉淀, 免疫印迹
宿主 :
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
BDNF
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Mouse, rat - 16/17 amino acid residues identical.
靶标/背景信息 :
Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF), neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5). All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways. Until recently, the functional role of the neurotrophin prodomains were thought to include assistance in the correct folding of the mature protein and the sorting of the neurotrophins into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions. The functional importance of the prodomain of BDNF was recently demonstrated in a study showing that a polymorphism that replaces valine for methionine at position 66 of the prodomain, is associated with memory defects and abnormal hippocampal function in humans. Another recent study showed that the regulated extracellular cleavage of proBDNF to mature BDNF by plasmin is necessary for establishing late-phase long-term potentiation (L-LTP), a process that involves long-lasting changes in the structure and function of hippocampal synapses. Finally, proBDNF was shown to be decreased in the brains of patients suffering from Alzheimer’s disease. Mature BDNF binds to the specific tyrosine kinase receptor TrkB and to p75NTR, a member of the TNF receptor superfamily. proBDNF can similarly bind both receptors although it appears to have a greater affinity for the p75NTR receptor.
免疫原 :
合成肽
区域 :
intracellular
Loop :
Pro-domain of the BDNF protein
序列 :
(C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor)
序列文本 :
Peptide (C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor) (Accession P23560). Pro-domain of the BDNF protein.
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.8毫克/毫升
浓度 :
08毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P23560
Top Related :
蚂蚁-010, B-250, AGP-021, B-257, AN-240
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
更多信息或购买 :
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年,在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。