抗体

抗p75神经生长因子受体(细胞外)抗体

蚂蚁-007

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

蚂蚁-007

Primary Antibody>Extracellular>Non-Labeled

Insert

抗p75神经生长因子受体(细胞外)抗体

Anti-p75 NGF Receptor (extracellular) Antibody (#ANT-007) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunoprecipitation, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize p75NTR from human, mouse and rat samples.

A兔多克隆抗体人类神经营养因子受体p75NTR

BLP-NT007

p75 NGF Receptor, p75NTR, p75 Neurotrophin receptor, Low affinity NGF receptor, TNFR superfamily member 16, CD271, NGFR

人类神经营养因子受体p75NTR

Neurotrophic Factors and Receptors > Neurotrophin Receptors and Co-Receptors > Antibodies to Receptors and Co-Receptors

未共轭

免疫细胞化学, IF, IFC, 免疫组化, 免疫沉淀, LCI, 免疫印迹

兔

多克隆

兔IgG

神经生长因子受体

人类(试验);小鼠(试验);大鼠(试验)

Mouse - identical; rat - 15/16 amino acid residues identical.

The p75 neurotrophin receptor (p75NTR) is a member of the TNF receptor superfamily. p75NTR, like all the superfamily members is a type I transmembrane protein with tandem cysteine-rich domains in the extracellular portion and a intracellular death domain. p75NTR binds its ligands as a homodimer but can also form heterodimers with other receptors such as TrkA, TrkB, TrkC, Nogo receptor and sortilin. The precise multimeric receptor complex formed between p75NTR and the other receptors will determine the ligand being recognized (see below) and the biological response to its binding. As it name implies, p75NTR binds all the neurotrophins (NGF, BDNF, NT-3 and NT-4) with similar nM affinities. Co-expression of p75NTR with the Trk receptors enhances the ability to bind and respond to the specific neurotrophin and induces cell survival. On the other hand, it has recently been demonstrated that p75NTR binds with high affinity to the unprocessed form of NGF (proNGF) probably as a complex with sortilin and this leads to cell death by apoptosis. Finally, a multimeric complex of p75NTR and Nogo receptor binds myelin proteins such as Nogo-66, MAG and OmgP resulting in inhibition of axonal growth.

合成肽

细胞外

Extracellular, stalk domain

CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75NTR

Peptide CEEIPGRWITRSTPPE, corresponding to amino acid residues 188-203 of human p75NTR (Accession P08138). Extracellular, stalk domain.

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P08138

蚂蚁-011, 蚂蚁-019, 蚂蚁-018, 蚂蚁-010, B-250

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

1

25

mcl

425美元

Cell surface detection of p75NTR by indirect flow cytometry in live intact rat PC12 pheochromocytoma cells: Cell surface detection of p75NTR by indirect flow cytometry in live intact rat PC12 pheochromocytoma cells: Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells + Anti-p75 NGF Receptor (extracellular) Antibody (#ANT-007), (2.5μg) + goat-anti-rabbit-FITC.

Immunoprecipitation of 3T3/p75NTR transfected cells: - 1. Cell lysate + protein A beads.2. Cell lysate + protein A beads + pre-immune rabbit serum.3. Cell lysate + protein A beads + Anti-p75 NGF Receptor (extracellular) Antibody (#ANT-007).4. Cell lysate.Red arrows indicate NGFR p75 while the black arrow shows the IgG heavy chain.Immunoblot was performed with the Anti-p75 NGF Receptor (extracellular) Antibody.