产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody
目录 :
蚂蚁-018
规格 :
1 X 50 mcl
价格 :
735美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫组化, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Alomone Labs ANT-018-FR 图像 1
Expression of TrkA in rat dorsal root ganglion - Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody (#ANT-018-FR), (1:50). TrkA (purple staining) is expressed in DRG neurons. Hoechst 33342 (blue) is used as the counterstain.
产品信息
SKU号 :
蚂蚁-018
ParentSKU :
蚂蚁-018
Taxonomy Node :
Primary Antibody>Extracellular>Labeled
Action :
Insert
产品名称 :
Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody
产品描述 :
Anti-TrkA (extracellular) Antibody (#ANT-018) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize TrkA from mouse, rat, and human samples. nAnti-TrkA (extracellular)-ATTO Fluor-633 Antibody (#ANT-018-FR) is directly labeled with an ATTO-633 fluorescent dye. ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. ATTO 633 has a maximum absorption at 629 nm and a maximum fluorescence at 657 nm. The fluorescence is excited most efficiently in the range 610 to 645 nm. This label is analogous to the well-known dyes Alexa 647, Alexa 633 and Cy5. Anti-TrkA (extracellular)-ATTO Fluor-633 Antibody is especially suited for experiments requiring simultaneous labeling of different markers.
Short Description :
A Rabbit Polyclonal Antibody to TrkA Conjugated to the Fluorescent Dye ATTO-633
Negative Control :
BLP-NT018
同义词 :
TrkA, High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, NTRK1
靶标名 :
TrkA
Primary Category (Old) :
Neurotrophic Factors and Receptors > Neurotrophin Receptors and Co-Receptors > Antibodies to Receptors and Co-Receptors
标记/共轭标签 :
ATTO 633 (Red)
应用 :
IF, 免疫组化
宿主 :
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
NTRK1
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Mouse - identical; human - 13/14 amino acid residues identical.
靶标/背景信息 :
NGF was the first neurotrophin to be discovered more than 50 years ago. Three other proteins have been added to the list: BDNF, NT-3 and NT-4 (NT-4/5). These neurotrophins bind two groups of receptors. The p75NTR receptor is common to all four neurotrophins and is a member of the tumor necrosis factor receptor family. The tropomyosin-related kinase (TrK) receptors are receptor tyrosine kinases (RTKs) and three receptors form this family: TrkA, TrkB, and TrkC1. As mentioned, the p75NTR receptor binds to all neurotrophins with similar affinities while the Trk receptors display selectivity for the neurotrophins. TrkA is activated by NGF binding, TrkB by that of BDNF and NT-4, while TrkC is stimulated by the binding of NT-31. All three Trk receptors are highly expressed in the mammalian brain in very distinct regions and are also expressed in the peripheral nervous system2-4. Cholinergic neurons in the basal forebrain exclusively express TrkA. All three Trk receptors are expressed in the hippocampus. Motor and sensory neurons in the peripheral nervous system express Trk receptors. Interestingly, while Trk receptors are not essential for development, knockout mice die shortly after birth. Indeed, TrkA-deficient mice demonstrate a significant decrease in cholinergic and sympathetic neurons in the trigeminal, superior cervical and dorsal root ganglia5-7. Trk receptors are type I membrane proteins (i.e. span the membrane with one transmembrane region) and have many motifs in the extracellular region, including cell-adhesion domains, three tandem leucine rich motifs flanked by two clusters of cysteines. In the membrane proximal region of the receptor there are also two immunoglobulin-like domains8. The intracellular region of the protein contains the kinase domain, responsible for activating downstream effectors as well as many adaptor site regions important for the binding/interaction of signaling molecules. The binding of neurotrophins to Trk receptors promotes receptor dimerization resulting in kinase activation. Activated Trk receptors then phosphorylate a cascade of signaling molecules including the Ras/ERK, PI3K/Akt pathways and PLC-γ1. Activated Trk receptors also create internal docking sites for other signaling adaptor proteins to bind8. Splice variants of TrkA, TrkB and TrkC have been observed. These splice isoforms are mainly effected in the tyrosine kinase domain of the receptor lying in the cytoplasm. Endocytosis is an important signaling trait of Trk receptors. Following neurotrophin binding to the Trk receptor, the receptor complex is then internalized via endocytosis in order to terminate signaling. However, in the axonal compartment of neurons, the internalization process of the neurotrophin complexed to the receptor is part of the signaling process and is important for activating transcription processes in the nucleus9,10.
免疫原 :
合成肽
区域 :
细胞外
Loop :
Extracellular, 2nd immunoglobulin-like domain
序列 :
(C)ETMRHG(S)LRLNQPTH, corresponding to amino acid residues 342-356 of rat TrkA
序列文本 :
Peptide (C)ETMRHG(S)LRLNQPTH, corresponding to amino acid residues 342-356 of rat TrkA (Accession P35739). Extracellular, 2nd immunoglobulin-like domain.
Primary Size (Size+Units) :
1 X 50 mcl
浓度 :
1毫克/毫升
浓度 :
1毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P35739
Top Related :
蚂蚁-007, 蚂蚁-007
储存和重组 :
Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).
包装规格 :
1
产品规格/规格 :
50
单位 :
mcl
标价美国[美元] :
735美元
更多信息或购买 :
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年,在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。