抗体

Anti-Ret (extracellular) Antibody

蚂蚁-025

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

蚂蚁-025

Primary Antibody>Extracellular>Non-Labeled

Insert

Anti-Ret (extracellular) Antibody

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of human Ret. Anti-Ret (extracellular) Antibody (# ANT-025) can be used in western blot, indirect flow cytometry, immunohistochemistry and immunocytochemistry applications and has been designed to recognize Ret from rat, mouse and human samples.

A Rabbit Polyclonal Antibody to Ret Receptor

BLP-NT025

Ret, Proto-oncogene tyrosine-protein kinase receptor Ret, Proto-oncogene c-Ret, Cadherin family member 12, CDHF12

Ret Receptor

Neurotrophic Factors and Receptors > Glial-Derived Neurotrophic Factor (GDNF) Family Receptors > Antibodies

未共轭

免疫细胞化学, IF, IFC, 免疫组化, LCI, 免疫印迹

兔

多克隆

兔IgG

Ret

人类(试验);小鼠(试验);大鼠(试验)

Mouse - identical; rat - 12/13 amino acid residues identical.

The RET proto-oncogene on human chromosome 10q11.2, encodes a receptor tyrosine kinase (RTK) activated by members of the glial cell line-derived neurotrophic factor (GDNF) ligand family (GDNF, neurturin, artemin, and persephin) in conjunction with a ligand-specific coreceptor (GFRα1-4)1. RET belongs to the cadherin superfamily, and it has been suggested that the ret gene is the result of a recombination between a gene encoding a tyrosine kinase receptor and a gene encoding an ancestral cadherin at an early stage of evolution2. The extracellular domain of RET comprises, as observed for other tyrosine kinase receptors, a cysteine-rich domain, but also four cadherin-like domains3. RET signaling is crucial for the development of the enteric nervous system. RET also regulates the development of sympathetic, parasympathetic, motor, and sensory neurons, and is necessary for the postnatal maintenance of dopaminergic neurons. Outside the nervous system, RET is crucial for development of the kidney and plays a key role in spermatogenesis3. RET has attracted considerable clinical interest because of the range of mutations found in diverse conditions that include Hirschsprung disease and a variety of cancers involving the thyroid gland. RET-related cancers with thyroid involvement include sporadic and familial medullary thyroid carcinoma (MTC), multiple endocrine neoplasia 2 (MEN2) syndromes MEN2A and MEN2B, and papillary thyroid carcinoma (PTC)4.

合成肽

细胞外

细胞外, N末端

CEWRQGDGKGITR, corresponding to amino acid residues 541-553 of human Ret

Peptide CEWRQGDGKGITR, corresponding to amino acid residues 541-553 of human Ret (Accession P07949). Extracellular, N-terminus.

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P07949

蚂蚁-021, 蚂蚁-014, 蚂蚁-018, G-240, 蚂蚁-023

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元

Cell surface detection of Ret by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:  Cell surface detection of Ret by indirect flow cytometry in live intact human THP-1 monocytic leukemia cell line:  Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells + Anti-Ret (extracellular) Antibody (#ANT-025), (5μg) + goat-anti-rabbit-FITC.

Expression of Ret in human MCF-7 breast adenocarcinoma cells - Cell surface detection of Ret in live intact human MCF-7 breast adenocarcinoma cells. A. Extracellular staining of cells with Anti-Ret (extracellular) Antibody (#ANT-025), (1:25), followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of the two images.