抗体

抗人类肿瘤坏死因子受体I(细胞外)抗体

蚂蚁-041

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

蚂蚁-041

Primary Antibody>Extracellular>Non-Labeled

Insert

抗人类肿瘤坏死因子受体I(细胞外)抗体

Anti-Human TNF Receptor I (extracellular) Antibody (#ANT-041) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. The antibody recognizes an extracellular epitope and can thus detect the protein in living cells. It has been designed to recognize TNFR1 from human samples only.

A兔多克隆抗体肿瘤坏死因子受体1

BLP-NT041

TNF Receptor I, Tumor necrosis factor receptor 1, TNFR1, TNFRSF1A, TNF receptor superfamily member 1A, CD120a

肿瘤坏死因子受体1

Neurotrophic Factors and Receptors > Cytokines and Receptors > Cytokines and Receptors > Antibodies

未共轭

IFC, LCI, 免疫印迹

兔

多克隆

兔IgG

肿瘤坏死因子受体超家族成员1A

人类(试验)

抗体识别只有人类TNFR1

Tumor necrosis factor (TNF) has a wide range of biological effects in host defense against pathogens. It is capable of inducing cell survival, proliferation and differentiation as well as both apoptotic and necrotic cell death under certain conditions. The TNFR1 receptor belongs to a family of type I transmembrane proteins. TNFR1, as well as other members of its family, is an elongated molecule composed of an extracellular domain of multiple ~40-residue pseudo repeats, with six cysteines forming three disulfide bridges. These modules are termed CRDs (cysteine-rich domains). Unlike many receptors, TNFR1 does not have enzymatic activity. Instead, an oligomeric complex, called the death domain (DD) places the intracellular regions of the receptor in proximity for recruitment of signaling proteins with enzymatic activities to amplify the signal transduction and mediate extrinsic signal induced cell death1. TNFα, a ligand of TNFR1 binds to the intracellular death domain of TNFR1 and recruits the TNF receptor-associated DD protein (TRADD), which in turn recruits receptor-interacting protein kinase 1 (RIP1), cellular inhibitor of apoptosis proteins 1 and 2 (cIAP1 and 2), and TNF receptor-associated factor 22. TNFR1-mediated signaling provides potent anti-viral activity, however many viruses have evolved to encode and express molecules that specifically inhibit almost every step of TNFR-induced apoptosis and NF-κB signaling pathways3.

合成肽

细胞外

细胞外, N末端

CRKNQYRHYWSEN, corresponding to amino acid residues 127-139 of human TNFR1

Peptide CRKNQYRHYWSEN, corresponding to amino acid residues 127-139 of human TNFR1 (Accession P19438). Extracellular, N-terminus

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P19438

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元

Cell surface detection of TNFR1 in live intact human MEG-01 megakaryoblastic leukemia cells: Cell surface detection of TNFR1 in live intact human MEG-01 megakaryoblastic leukemia cells: Black line: Cells. Red line: Cells + Goat-anti-Rabbit-FITC. Green line: Cells + Anti-Human TNF Receptor I (extracellular) Antibody (#ANT-041), 5 µg + Goat-anti-Rabbit-FITC.