抗体

Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody

ANX-033

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

ANX-033

Primary Antibody>Intracellular>Non-Labeled

Insert

Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat NHE-3. Anti-Na+/H+ Exchanger 3 (NHE-3) Antibody (#ANX-033) can be used in western blot analysis. It has been designed to recognize NHE-3 from human, mouse and rat samples.

A Rabbit Polyclonal Antibody to Na+/H+ Exchanger 3 (NHE-3)

BLP-NX033

Na+/H+ Exchanger 3 (NHE-3), Sodium/hydrogen exchanger 3, SLC9A3

Na+/H+ Exchanger 3 (NHE-3)

Transporters , Exchangers and Pumps > Na+/H+ Exchangers > Antibodies

未共轭

免疫印迹

兔

多克隆

兔IgG

SLC9A3

Human (Tested);Rat (Tested);Mouse (Predicted)

Mouse – 13/14 amino acid residues identical; human – 12/14 amino acid residues identical.

Maintaining physiological intracellular pH values is vital for most cells. The Na+/H+ exchanger family (NHE, also known as SLC9) is widely expressed and has a key role in protecting cells against acidification. There are currently six NHE transporters identified in mammalian cells1. The complete structure of members of the NHE family has not been completely solved. However, all members are organized in a similar manner with 11 or 12 membrane spanning domains, carrying out the Na+ and H+ exchange and a varying length intracellular C-terminal domain being involved in regulation of the exchange activity. The transport of Na+ and H+ in NHEs occurs in the center of the N-terminus via a two inverted funnel area that is made up of two transmembrane regions. NHE3 is expressed in sodium absorptive cells of the mammalian small intestine, colon, gall bladder, renal proximal tubule, and thick and thin limbs of the loop of Henle and can be mostly found in the plasma membrane2. NHE3 interacts with a multi-PDZ domain-containing scaffold protein called NHERF-1. NHERF-1 binds to the NHE3 C-terminus and has a role in NHE3 regulation in various cells. NHERF-1 functions as a cofactor required for the phosphorylation and downregulation of NHE3 activity in response to elevated intracellular cAMP3. In addition, silencing of NHERF-1 in a model of diabetic mice was found to prevent NHE3 trafficking and insulin-dependent activation4.

合成肽

intracellular

Intracellular, C-terminus

(C)EQREPKLNEKLHGR, corresponding to amino acid residues 466-479 of rat NHE-3

Peptide (C)EQREPKLNEKLHGR, corresponding to amino acid residues 466-479 of rat NHE-3 (Accession P26433). Intracellular, C-terminus.

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P26433

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元