抗体

抗KCNJ1(Kir1.1)抗体

APC-001

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀

APC-001

Primary Antibody>Intracellular>Non-Labeled

Insert

抗KCNJ1(Kir1.1)抗体

Anti-KCNJ1 (Kir1.1) Antibody (#APC-001) is a highly specific antibody directed against an epitope of the rat potassium channel ROM-K. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and immunoprecipitation applications. It has been designed to recognize ROMK1 from rat, mouse, and human samples.

A Rabbit Polyclonal Antibody to KCNJ1 (Kir1.1) Channel

BLP-PC001

KCNJ1 (Kir1.1), ROMK1, ATP-sensitive inward rectifier potassium channel 1

KCNJ1 (Kir1.1) Channel

Ion Channels > K+ Channels > Inward Rectifier K+ Channels > Antibodies to Kir Channels

未共轭

免疫细胞化学, IF, 免疫组化, 免疫沉淀, 免疫印迹

兔

多克隆

兔IgG

KCNJ1

人类(试验);小鼠(试验);大鼠(试验)

Mouse - identical; human - 45/50 amino acid residues identical.

Kir1.1 (KCNJ1, ROMK1) was the first member of the family of inward rectifying K+ channels to be cloned.1 The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels. The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels, the functional unit for the Kir channel is composed of four subunits that can assemble as either homo or heterotetramers. Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.3 As its original name indicates (ROMK1, Renal Outer Medullary K+ channel), Kir1.1 is strongly expressed in the kidney in the apical membrane of several kidney segments such as the thick ascending loop of Henle (TAL) and the cortical collecting duct (CCD). In addition, the channel is also expressed in the brain, mainly in the cortex and hippocampus.3 Kir1.1 plays a key role in K+ recycling in the loop of Henle. Indeed, loss-of-function mutations in the Kir1.1 gene cause Bartter’s syndrome type II, a recessive autosomal disease characterized by the impairment of K+ efflux and the subsequent inability of the NKCC2 transporter to continue NaCl uptake. This leads to a high salt concentration in the urine that induces osmotic diuresis and low plasma volume.2  Pharmacologically, the Kir1.1 channel can be inhibited by several general K+ channel blockers such as Tertiapin (#STT-250), however the scorpion toxin Lq2 (#RTL-550) specifically and potently inhibits Kir1.1 channels.4

谷胱甘肽S-转移酶融合蛋白

intracellular

Intracellular, C-terminus

DDTQM, corresponding to amino acids 342-391 of rat KCNJ1
HNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVL
SEVDET
GST fusion protein with the sequence

DDTQM, corresponding to amino acids 342-391 of rat KCNJ1 (Accession P35560). Intracellular, C-terminus.
HNFGKTVEVETPHCAMCLYNEKDARARMKRGYDNPNFVL
SEVDET
GST fusion protein with the sequence

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen

-20°C

P35560

ACL-004, ASC-019, APC-159, APC-027, APC-006

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元