抗体

抗Kv1.2(KCNA2)抗体

APC-010

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

APC-010

Primary Antibody>Intracellular>Non-Labeled

Insert

抗Kv1.2(KCNA2)抗体

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KV1.2. Anti-KV1.2 (KCNA2) Antibody (#APC-010) can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KV1.2 from human, rat, and mouse samples.

A Rabbit Polyclonal Antibody to KV1.2 (KCNA2) Channel

BLP-PC010

KV1.2 (KCNA2), Potassium voltage-gated channel subfamily A member 2, RBK2

KV1.2 (KCNA2) Channel

Ion Channels > K+ Channels > Voltage-Gated K+ Channels > Antibodies to KV Channels

未共轭

免疫细胞化学, IF, 免疫组化, 免疫印迹

兔

多克隆

兔IgG

KCNA2

人类(试验);小鼠(试验);大鼠(试验)

Mouse, dog, human, - identical.

KV1.2 is a mammalian voltage-dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.2 was first cloned from rat brain.1 Eight Shaker-related genes exist in mammals constituting the KV1 subfamily of the large KV channel family of genes.2 A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function. The structure of KV1.2 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2 The channel is expressed in neurons and cardiac and smooth muscle tissue as well as in retina and pancreas.2 The crystal structure of KV1.2 was recently solved shading light on the structure of a mammalian voltage dependent channel.3 The functional channel is considered low voltage activated and shows very little inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle. KV1.2 channels are sensitive to high doses of TEA (560 mM) and low doses of 4-AP (0.59 mM), the “classical” non-selective potassium channel blockers. Several venomous toxins from snakes, scorpions and honey bee are potent blockers (affecting the channels in the nanomolar range) of KV1.2 channels. Among these, the most potent and selective are α-Dendrotoxin (1-12 nM), Dendrotoxin-I (0.13 nM), Maurotoxin (0.1-0.8 nM), Hongotoxin-1 (0.17 nM), Margatoxin (0.16-0.65 nM), Tityustoxin Kα (0.21 nM) and MCD peptide (10-400 nM).4

谷胱甘肽S-转移酶融合蛋白

intracellular

Intracellular, C-terminus

corresponding to amino acid residues 417-499 of rat KV1.2
SRSASTISKSDYMEIQEGVNNSNEDFREENLKTANCTLA
NTNYVNITKMLTDV,
GST fusion protein with the sequence YHRETEGEEQAQYLQVTSCPKIPSSPDLKK

corresponding to amino acid residues 417-499 of rat KV1.2 (Accession P63142). Intracellular, C-terminus.
SRSASTISKSDYMEIQEGVNNSNEDFREENLKTANCTLA
NTNYVNITKMLTDV,
GST fusion protein with the sequence YHRETEGEEQAQYLQVTSCPKIPSSPDLKK

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 5% sucrose, 0.025% NaN3.

冻干粉末

The serum was depleted of anti-GST antibodies by affinity chromatography on immobilized GST and then the IgG fraction was purified on immobilized antigen

-20°C

P63142

APC-009, ASC-009, APC-161, APC-003, APC-012

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元