产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗Kv1.3(KCNA3)(细胞外)抗体
目录 :
APC-101
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀
更多信息或购买 :
Alomone Labs 产品网页
文章摘录数: 10
出版应用/物种/样本/稀释参考文献
  • 免疫印迹; 小鼠; 1:400; 图 4a
Angelova D, Brown D. Model Senescent Microglia Induce Disease Related Changes in α-Synuclein Expression and Activity. Biomolecules. 2018;8: pubmed 出版商
Shi S, Zhao Q, Ke C, Long S, Zhang F, Zhang X, et al. Loureirin B Exerts its Immunosuppressive Effects by Inhibiting STIM1/Orai1 and KV1.3 Channels. Front Pharmacol. 2021;12:685092 pubmed 出版商
Peruzzo R, Mattarei A, Azzolini M, Becker Flegler K, Romio M, Rigoni G, et al. Insight into the mechanism of cytotoxicity of membrane-permeant psoralenic Kv1.3 channel inhibitors by chemical dissection of a novel member of the family. Redox Biol. 2020;37:101705 pubmed 出版商
Nguyen H, Di Lucente J, Chen Y, Cui Y, Ibrahim R, Pennington M, et al. Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4-mediated calcium entry in microglia. Glia. 2020;68:2377-2394 pubmed 出版商
Peruzzo R, Mattarei A, Romio M, Paradisi C, Zoratti M, Szabo I, et al. Regulation of Proliferation by a Mitochondrial Potassium Channel in Pancreatic Ductal Adenocarcinoma Cells. Front Oncol. 2017;7:239 pubmed 出版商
Rangaraju S, Raza S, Pennati A, Deng Q, Dammer E, Duong D, et al. A systems pharmacology-based approach to identify novel Kv1.3 channel-dependent mechanisms in microglial activation. J Neuroinflammation. 2017;14:128 pubmed 出版商
Leanza L, Romio M, Becker K, Azzolini M, Trentin L, Managò A, et al. Direct Pharmacological Targeting of a Mitochondrial Ion Channel Selectively Kills Tumor Cells In Vivo. Cancer Cell. 2017;31:516-531.e10 pubmed 出版商
Vivekananda U, Novak P, Bello O, Korchev Y, Krishnakumar S, Volynski K, et al. Kv1.1 channelopathy abolishes presynaptic spike width modulation by subthreshold somatic depolarization. Proc Natl Acad Sci U S A. 2017;114:2395-2400 pubmed 出版商
Zaccagnino A, Managò A, Leanza L, Gontarewitz A, Linder B, Azzolini M, et al. Tumor-reducing effect of the clinically used drug clofazimine in a SCID mouse model of pancreatic ductal adenocarcinoma. Oncotarget. 2017;8:38276-38293 pubmed 出版商
Jiménez Pérez L, Cidad P, Álvarez Miguel I, Santos Hipólito A, Torres Merino R, Alonso E, et al. Molecular Determinants of Kv1.3 Potassium Channels-induced Proliferation. J Biol Chem. 2016;291:3569-80 pubmed 出版商
图像
图像 1 :
Alomone Labs APC-101 图像 1
Western blot analysis of rat brain membranes:   - 1. Anti-KV1.3 (KCNA3) (extracellular) Antibody  (#APC-101), (1:500).  2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).
图像 2 :
Alomone Labs APC-101 图像 2
Peptide KDYPASTSQDSFEA(C) corresponding to amino acid residues 263-276 of human KV1.3 (AccessionP22001). 1stextracellular loop.
产品信息
SKU号 :
APC-101_1_25_mcl
ParentSKU :
APC-101
Taxonomy Node :
Primary Antibody>Extracellular>Non-Labeled
Action :
Insert
产品名称 :
抗Kv1.3(KCNA3)(细胞外)抗体
产品描述 :
Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize KV1.3 potassium channel from human, mouse and rat samples.
Short Description :
A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel
Negative Control :
BLP-PC101
同义词 :
KV1.3 (KCNA3), Potassium voltage-gated channel subfamily A member 3
靶标名 :
KV1.3 (KCNA3) Channel
Primary Category (Old) :
Ion Channels > K+ Channels > Voltage-Gated K+ Channels > Antibodies to KV Channels
标记/共轭标签 :
未共轭
应用 :
免疫细胞化学, IF, IFC, 免疫组化, 免疫沉淀, LCI, 免疫印迹
宿主 :
兔
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
KCNA3
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Rat, mouse - 12/14 amino acid residues identical.
靶标/背景信息 :
KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. The prominence of KV1.3 channels in these cells (particularly in T lymphocytes) directed much research attention. It was found that KV1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2 Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.1 The central role of KV1.3 in immune system cells created a real need for a specific antibody that would be able to work in flow cytometry applications.
免疫原 :
合成肽
区域 :
细胞外
Loop :
Extracellular loop between domains S1 and S2.
序列 :
KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3
序列文本 :
Peptide KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3 (Accession P22001). Extracellular loop between domains S1 and S2.
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.8毫克/毫升
浓度 :
08毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P22001
Top Related :
APC-002, APC-101-F, APC-161, STM-325, STS-400
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
IFC Legend :
Cell surface detection of KV1.3 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells: Cell surface detection of KV1.3 by indirect flow cytometry in live intact human Jurkat T-cell leukemia cells: Black line: Cells. Red line: Cells + goat-anti-rabbit-APC. Green line: Cells + Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (5μg) + goat-anti-rabbit-APC.
LCI, ICC Legend :
Expression of KV1.3 in HEK 293 transfected cells - Cell surface detection of KV1.3 in live intact HEK 293 cells transfected with rat KV1.3 (panels A and C) or with empty vector (panels B and D) using Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:50).Staining (green) shows expression of KV1.3 in the transfected cells (A) but not in the cells transfected with empty vector (B). Panels C and D show the corresponding live image of the cells.
更多信息或购买 :
Alomone Labs 产品网页
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年，在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。