抗体

抗Kir2.1/KCNJ2(细胞外)抗体

APC-159

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

APC-159

Primary Antibody>Extracellular>Non-Labeled

Insert

抗Kir2.1/KCNJ2(细胞外)抗体

Anti-Kir2.1/KCNJ2 (extracellular) Antibody (#APC-159) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, live cell imaging and live cell flow cytometry. It has been designed to recognize Kir2.1 from human, rat, and mouse samples.

A Rabbit Polyclonal Antibody to Kir2.1 (KCNJ2) Channel

BLP-PC159

Kir2.1(KCNJ2), 内向整流钾离子通道2, IRK1, HIRK1, LQT7, SQT3

Kir2.1 (KCNJ2) Channel

Ion Channels > K+ Channels > Inward Rectifier K+ Channels > Antibodies to Kir Channels

未共轭

免疫细胞化学, IF, IFC, 免疫组化, LCI, 免疫印迹

兔

多克隆

兔IgG

KCNJ2

人类(试验);小鼠(试验);大鼠(试验)

Rat, dog, pig - 13/14 amino acid residues identical; human - 12/14 amino acid residues identical; mouse - 11/14 amino acid residues identical.

Inwardly rectifying K+ (Kir) channels allow K+ to move more easily in, rather than out, of the cell. They have diverse physiological functions depending on their type and their location. There are seven Kir channel subfamilies that can be classified into four functional groups. Kir2.1 belongs to the Kir2.x subfamily. The primary structure of Kir2.1 is comprised of two putative membrane-spanning domains (TM1 and TM2) linked by an extracellular pore-forming region (H5) and cytoplasmic amino and carboxy terminal domains. The H5 region serves as the “ion-selectivity filter” that is shared with other K+-selective ion channels with the signature sequence T-X-G-Y(F)-G. Although initially thought to form only homomeric complexes, it is now known that Kir2.1 can function as a heterotetramer with other Kir2.x subunits both in vitro and in vivo.  After synthesis in the endoplasmic reticulum, Kir2.1 is transported to the Golgi apparatus for post-translational modification, and then transported to the cell surface. Sequences in the NH2-terminal region have been identified as responsible for Golgi export. Kir2.1 is expressed in skeletal and cardiac muscle and contributes to the establishment of highly negative resting potential and long-lasting action potential plateau in various cells including cardiac myocytes. Inward rectification of Kir2.1 is caused by intracellular ions such as Mg2+ and polyamines. Andersen syndrome is caused by dysfunction of Kir2.1. Patients with Andersen syndrome exhibit cardiac arrhythmias reminiscent of long Q-T syndrome, periodic paralysis, and dysmorphic bone structure in the face and fingers.

合成肽

细胞外

Extracellular loop

(C)DLDASKESKA(S)VSE, corresponding to amino acid residues 112 - 125 of rat Kir2.1

Peptide (C)DLDASKESKA(S)VSE, corresponding to amino acid residues 112 - 125 of rat Kir2.1 (Accession Q64273). Extracellular loop.

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

Q64273

APC-026, APC-017, ACC-003, ASC-005, APC-001

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元

Cell surface detection of Kir2.1 in live intact human THP-1 monocytic leukemia cells: Cell surface detection of Kir2.1 in live intact human THP-1 monocytic leukemia cells: Black line: Cell alone. Red line: Cells + goat-anti-rabbit-Alexa-488. Green line: Cells + Anti-Kir2.1/KCNJ2 (extracellular) Antibody (#APC-159), 2.5µg + goat-anti-rabbit-AlexaFluor-488 secondary antibody.

Expression of Kir2.1 in rat RBL-2H3 cells - Cell surface detection of Kir2.1 in live intact rat RBL-2H3 basophilic leukemia cells. A. Extracellular staining of cells using Anti-Kir2.1/KCNJ2 (extracellular) Antibody (#APC-159), (1:50), followed by goat-anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells.