产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
Anti-KCNE1 (IsK) Antibody
目录 :
APC-163
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹
更多信息或购买 :
文章摘录数: 3
参考文献
Cechmanek P, Hehr C, McFarlane S. Retinal Pigment Epithelium and Neural Retinal Progenitors Interact via Semaphorin 6D to Facilitate Optic Cup Morphogenesis. Eneuro. 2021;8: pubmed 出版商
Strigli A, Raab C, Hessler S, Huth T, Schuldt A, Alzheimer C, et al. Doxorubicin induces caspase-mediated proteolysis of KV7.1. Commun Biol. 2018;1:155 pubmed 出版商
Lv Y, Wang Y, Zhu X, Zhang H. Aldosterone downregulates delayed rectifier potassium currents through an angiotensin type 1 receptor-dependent mechanism. Am J Transl Res. 2018;10:1413-1421 pubmed
图像
图像 1 :
Alomone Labs APC-163 图像 1
Western blot analysis of rat kidney (lanes 1 and 4), rat heart (lanes 2 and 5) and mouse kidney (lanes 3 and 6) membranes: - 1-3. Anti-KCNE1 (IsK) Antibody (#APC-163), (1:200).4-6. Anti-KCNE1 (IsK) Antibody, preincubated with KCNE1/IsK Blocking Peptide (#BLP-PC163).
图像 2 :
Alomone Labs APC-163 图像 2
Western blot analysis of rat kidney (lanes 1 and 4) rat heart (lanes 2 and 5) and mouse kidney (lanes 3 and 6) membranes: - 1-3.Anti-KCNE1 (IsK) Antibody (#APC-163) (1:200).4-6. Anti-KCNE1 (IsK) Antibody preincubated with the negative control antigen.
图像 3 :
Alomone Labs APC-163 图像 3
Western blot analysis of human SH-SY5Y neuroblastoma (lanes 1 and 4) human HT-29 colon adenocarcinoma (lanes 2 and 5) and human MCF-7 breast adenocarcinoma (lanes 3 and 6)cell line lysates: - 1-3. Anti-NETO2 (extracellular) Antibody (#AGC-046) (1:200).4-6. Anti-NETO2 (extracellular) Antibody preincubated with the negative control antigen.
产品信息
SKU号 :
APC-163_1_25_mcl
ParentSKU :
APC-163
Taxonomy Node :
Primary Antibody>Intracellular>Non-Labeled
Action :
Insert
产品名称 :
Anti-KCNE1 (IsK) Antibody
产品描述 :
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KCNE1. Anti-KCNE1 (IsK) Antibody (#APC-163) can be used in western blot analysis. It has been designed to recognize KCNE1 from rat, mouse and human samples.
Short Description :
A兔多克隆抗体KCNE1
Negative Control :
BLP-PC163
同义词 :
KCNE1 (IsK), Delayed rectifier potassium channel subunit IsK, Minimal potassium channel, MinK
靶标名 :
KCNE1
Primary Category (Old) :
Ion Channels > K+ Channels > Voltage-Gated K+ Channels > Antibodies to KV Channels
标记/共轭标签 :
未共轭
应用 :
免疫印迹
宿主 :
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
KCNE1
反应物种 :
Mouse (Tested);Rat (Tested);Human (Predicted)
同源 :
Mouse, pig, dog – identical; human – 12/13 amino acid residues identical.
靶标/背景信息 :
Potassium delayed rectifier channels are a sub-type of potassium channels found in various species of animals. They are responsible for the late-repolarization phase and the duration of action potential, especially in cardiac myocytes. There are two types of delayed rectifier channels (Ik). The Fast component is known as Ikr and is encoded by the gene KCNH2 in Xenopus. This channel’s α-subunit is a 6-transmembrane domains voltage dependent potassium channel and its regulatory β-subunit, MiRP1, is required to reconstitute native Ikr1. KCNE1 and KCNQ1 are the genes encoding the two components of the Iks channel and this association was not fully understood for a long time. KCNQ1 (KV7.1) is a protein with six transmembrane domains and a typical pore loop containing a potassium signature sequence. The KNCE1 gene encodes the protein “minK”. This gene is one of family of genes that encode small channel subunits (smaller than 130 amino-acids) that co-assemble with bigger subunits thus changing channel gating properties. These are currently the smallest known potassium channel subunits. Their small size and putative transmembrane domain prevent them from forming channels on their own. In addition, in an experiment where mammalian cells were transfected with minK, no K+ currents were observed. When transfecting cells with a combination of KNCE1 and KNCQ1 the currents observed were up to ten times larger than currents induced by the KNCQ1 subunit alone2. The loss of function of Iks is tightly linked with the long QT syndrome and bilateral deafness and gain of function was found to be linked with cardiac tachy-arrhythmias3,4.
免疫原 :
合成肽
区域 :
intracellular
Loop :
Intracellular, C-terminus
序列 :
(C)RSKKLEHSHDPFN, corresponding to amino acid residues 68-80 of rat KCNE1
序列文本 :
Peptide (C)RSKKLEHSHDPFN, corresponding to amino acid residues 68-80 of rat KCNE1 (Accession P15383). Intracellular, C-terminus.
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.8毫克/毫升
浓度 :
08毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P15383
Top Related :
APC-022, APC-054, APC-062, APC-168, APC-026
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
更多信息或购买 :
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年,在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。