产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗Kir4.1(KCNJ10)(细胞外)抗体
目录 :
APC-165
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
文章摘录数: 5
参考文献
Gilbert A, Elorza Vidal X, Rancillac A, Chagnot A, Yetim M, Hingot V, et al. Megalencephalic leukoencephalopathy with subcortical cysts is a developmental disorder of the gliovascular unit. elife. 2021;10: pubmed 出版商
Huang B, Zdora I, de Buhr N, Lehmbecker A, Baumg xe4 rtner W, Leitzen E. Phenotypical peculiarities and species-specific differences of canine and murine satellite glial cells of spinal ganglia. J Cell Mol Med. 2021;25:6909-6924 pubmed 出版商
Schirmer L, Mobius W, Zhao C, Cruz Herranz A, Ben Haim L, Cordano C, et al. Oligodendrocyte-encoded Kir4.1 function is required for axonal integrity. elife. 2018;7: pubmed 出版商
Kelley K, Ben Haim L, Schirmer L, Tyzack G, Tolman M, Miller J, et al. Kir4.1-Dependent Astrocyte-Fast Motor Neuron Interactions Are Required for Peak Strength. Neuron. 2018;98:306-319.e7 pubmed 出版商
Cui Y, Yang Y, Ni Z, Dong Y, Cai G, Foncelle A, et al. Astroglial Kir4.1 in the lateral habenula drives neuronal bursts in depression. Nature. 2018;554:323-327 pubmed 出版商
图像
图像 1 :
Alomone Labs APC-165 图像 1
Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1, 2. Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165), (1:500).3, 4. Anti-Kir4.1 (KCNJ10) (extracellular) Antibody, preincubated with Kir4.1/KCNJ10 (extracellular) Blocking Peptide (#BLP-PC165).
图像 2 :
Alomone Labs APC-165 图像 2
Peptide (C)HGDLLELGPPANHT corresponding to amino acid residues 93-106 of rat Kir4.1 (AccessionP49655). Extracellular loop.
图像 3 :
Alomone Labs APC-165 图像 3
Expression of Kir4.1 in human U-87 MG cells - Cell surface detection ofKir4.1 in live intact human glioblastoma U-87 MG cells. A. Extracellular staining of cells usingAnti-Kir4.1(KCNJ10)(extracellular) Antibody (#APC-165) (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of A and B.
产品信息
SKU号 :
APC-165_1_25_mcl
ParentSKU :
APC-165
Taxonomy Node :
Primary Antibody>Extracellular>Non-Labeled
Action :
Insert
产品名称 :
抗Kir4.1(KCNJ10)(细胞外)抗体
产品描述 :
Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize Kir4.1 potassium channel from rat, mouse, and human samples.
Short Description :
A Rabbit Polyclonal Antibody to Kir4.1 (KCNJ10) Channel
Negative Control :
BLP-PC165
同义词 :
Kir4.1, ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel Inwardly rectifying subfamily J member 10
靶标名 :
Kir4.1 (KCNJ10) Channel
Primary Category (Old) :
Ion Channels > K+ Channels > Inward Rectifier K+ Channels > Antibodies to Kir Channels
标记/共轭标签 :
未共轭
应用 :
免疫细胞化学, IF, 免疫组化, LCI, 免疫印迹
宿主 :
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
KCNJ10
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Mouse - identical; human - 13/14 amino acid residues identical.
靶标/背景信息 :
Kir4.1 is a member of the inward rectifying K+ channel family. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels. The family’s topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heteromers. Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis. Kir4.1 is a member of the Kir4 subfamily that includes one other member: Kir4.2. Kir4.1 can co-assemble with Kir4.2 but also with other Kir channels such as Kir2.1 and Kir5.1. The Kir4 subfamily has been classified as weak rectifiers with intermediate conductance.  Kir4.1, encoded by KCNJ10, is mainly expressed in brain, specifically in glia cells, but also in retina, ear and kidney.1,2 It has been proposed that Kir4.1 has an essential role in glial K+ buffering, a process that re-uptakes the K+ released during neuronal activity into the intracellular interstitial space. Loss of Kir4.1 causes retinal defects and loss of endochoclear potential.3
免疫原 :
合成肽
区域 :
细胞外
Loop :
Extracellular loop
序列 :
(C)HGDLLELGPPANHT, corresponding to amino acid residues 93-106 of rat Kir4.1
序列文本 :
Peptide (C)HGDLLELGPPANHT, corresponding to amino acid residues 93-106 of rat Kir4.1 (Accession P49655). Extracellular loop.
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.8毫克/毫升
浓度 :
08毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P49655
Top Related :
APC-035, AQP-004, APC-035-AG, APC-125, AGP-012
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
LCI, ICC Legend :
Expression of Kir4.1 in human U-87 MG cells - Cell surface detection of Kir4.1 in live intact human glioblastoma U-87 MG cells. A. Extracellular staining of cells using Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165), (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of A and B.
更多信息或购买 :
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年,在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。