产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗Kv1.4(细胞外)抗体
目录 :
APC-167
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
文章摘录数: 2
参考文献
Alvarez P, Bogen O, Levine J. Interleukin 6 decreases nociceptor expression of the potassium channel KV1.4 in a rat model of hand-arm vibration syndrome. Pain. 2019;160:1876-1882 pubmed 出版商
Vivekananda U, Novak P, Bello O, Korchev Y, Krishnakumar S, Volynski K, et al. Kv1.1 channelopathy abolishes presynaptic spike width modulation by subthreshold somatic depolarization. Proc Natl Acad Sci U S A. 2017;114:2395-2400 pubmed 出版商
图像
图像 1 :
Alomone Labs APC-167 图像 1
Western blot analysis of rat brain lysate: - 1. Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:400).2. Anti-KV1.4 (extracellular) Antibody, preincubated with Kv1.4 (extracellular) Blocking Peptide (#BLP-PC167).
图像 2 :
Alomone Labs APC-167 图像 2
Expression of KV1.4 in rat parietal cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:400), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. KV1.4 staining (red) appears in several neuronal-outlined cells (arrows). Nuclei were stained with DAPI (blue).
产品信息
SKU号 :
APC-167_1_25_mcl
ParentSKU :
APC-167
Taxonomy Node :
Primary Antibody>Extracellular>Non-Labeled
Action :
Insert
产品名称 :
抗Kv1.4(细胞外)抗体
产品描述 :
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KV1.4. Anti-KV1.4 (extracellular) Antibody (#APC-167) can be used in western blot, immunohistochemistry and live cell imaging applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize KV1.4 from human, rat and mouse samples.
Short Description :
A Rabbit Polyclonal Antibody to KV1.4 Channel
Negative Control :
BLP-PC167
同义词 :
Kv1.4, Potassium voltage-gated channel subfamily A member 4, KCNA4
靶标名 :
KV1.4 Channel
Primary Category (Old) :
Ion Channels > K+ Channels > Voltage-Gated K+ Channels > Antibodies to KV Channels
标记/共轭标签 :
未共轭
应用 :
免疫细胞化学, IF, 免疫组化, LCI, 免疫印迹
宿主 :
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
KCNA4
反应物种 :
Mouse (Tested);Rat (Tested);Human (Predicted)
同源 :
Mouse – identical; human – 13/15 amino acid residues identical.
靶标/背景信息 :
KV1.4 is a mammalian voltage-dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.4 was first cloned from rat brain.1 Eight Shaker-related genes exist in mammals constituting the KV1 subfamily of the large KV channel family of genes.2 A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition, several auxiliary subunits and intracellular proteins might interact with the channel and affect its function. The structure of KV1.4 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2 The channel is expressed in neurons and cardiac and skeletal muscle tissue as well as in the pancreas.2 The functional channel is considered transient (A-type) current and shows pronounced inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle. KV1.4 channels are sensitive to high doses of TEA ( 100 mM) and low doses of 4-AP (0.013 mM), the “classical” non-selective potassium channel blockers. Most venomous peptide toxins that affect other KV channels do not inhibit KV1.4. However, the sea anemone toxin Stichodactyla Toxin (ShK), which is more potent towards KV1.1 and KV1.3, is still a potent inhibitor of KV1.4 channels.3
免疫原 :
合成肽
区域 :
细胞外
Loop :
1st extracellular loop
序列 :
(C)GHSRLLNDTSAPHLE, corresponding to amino acid residues 348 - 362 of rat KV1.4
序列文本 :
Peptide (C)GHSRLLNDTSAPHLE, corresponding to amino acid residues 348 - 362 of rat KV1.4 (Accession P15385). 1st extracellular loop.
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.8毫克/毫升
浓度 :
08毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P15385
Top Related :
APC-007, AGP-109, APC-101, APC-004, AGP-038
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
LCI, ICC Legend :
Expression of KV1.4 in live intact rat PC12 cells - Cell surface detection of KV1.4 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells with Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:25), followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.
更多信息或购买 :
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年,在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。