抗体

抗KCNQ1(细胞外)抗体

APC-168

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫细胞化学

APC-168

Primary Antibody>Extracellular>Non-Labeled

Insert

抗KCNQ1(细胞外)抗体

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of rat KCNQ1 (KV7.1). Anti-KCNQ1 (extracellular) Antibody (#APC-168) can be used in western blot and live cell imaging applications. It has been designed to recognize KCNQ1 from rat, human and mouse samples.

A Rabbit Polyclonal Antibody to KCNQ1 (KV7.1) Channel

BLP-PC168

KCNQ1, KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KCNA9, KvLQT1

KCNQ1 (KV7.1) Channel

Ion Channels > K+ Channels > Voltage-Gated K+ Channels > Antibodies to KV Channels

未共轭

免疫细胞化学, IF, IFC, LCI, 免疫印迹

兔

多克隆

兔IgG

KCNQ1

人类(试验);小鼠(试验);大鼠(试验)

Mouse – identical; human – 13/14 amino acid residues identical.

KCNQ1 (KV7.1) is part of a voltage-gated K+ family that includes 5 other members (KCNQ1-5). The channel has been intensively studied since it was found that together with MinK (Isk) it underlay the cardiac Iks current that controls the duration of the action potential of the human heart. Indeed mutations on either KV7.1 or MinK can be responsible for long QT syndrome, a cardiac disorder that causes arrhythmias and sudden death.  Although KV7.1 expression and function has been mainly investigated in relation with cardiac function the channel is widely distributed in epithelial tissues. There it has been implicated in physiological functions such as: regulation of acid secretion in the stomach and Cl- secretion into the colon.

合成肽

细胞外

3rd extracellular loop

(C)EKDAVNESGRIEFG, corresponding to amino acid residues 284-297 of rat KCNQ1

Peptide(C)EKDAVNESGRIEFG, corresponding to amino acid residues 284-297 of rat KCNQ1 (Accession Q9Z0N7). 3rd extracellular loop.

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

Q9Z0N7

APC-022, APC-109-F, APC-163, ACC-003, APC-016

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元

Cell surface detection of KCNQ1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:  Cell surface detection of KCNQ1 by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells:  Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells + Anti-KCNQ1 (extracellular) Antibody (#APC-168), (2.5μg) + goat-anti-rabbit-FITC.

Expression of KCNQ1 in Human COLO-205 cells - Cell surface detection of KCNQ1 in live intact human COLO-205 colon adenocarcinoma cells. A. Extracellular staining of cells with Anti-KCNQ1 (extracellular) Antibody (#APC-168), (1:50), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells. C. Merge of A and B.