产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗P2X7受体(细胞外)抗体
目录 :
APR-008
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 流式细胞仪, 免疫组化-石蜡切片, proximity ligation assay
更多信息或购买 :
Alomone Labs 产品网页
文章摘录数: 26
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 小鼠; 1:200; 图 3a
Campagno K, Lu W, Jassim A, Albalawi F, Cenaj A, Tso H, et al. Rapid morphologic changes to microglial cells and upregulation of mixed microglial activation state markers induced by P2X7 receptor stimulation and increased intraocular pressure. J Neuroinflammation. 2021;18:217 pubmed 出版商
  • proximity ligation assay; 小鼠; 图 4a
Rhodes G, Segars K, Lee Y, Hutcheon A, Rich C, Trinkaus Randall V. Pannexin1: Role as a Sensor to Injury Is Attenuated in Pretype 2 Corneal Diabetic Epithelium. Anal Cell Pathol (Amst). 2021;2021:4793338 pubmed 出版商
  • 免疫组化; 小鼠; 图 1
Jager S, Vaegter C. Avoiding experimental bias by systematic antibody validation. Neural Regen Res. 2016;11:1079-80 pubmed 出版商
  • 免疫组化-石蜡切片; 人类; 1:200; 图 4b
Giannuzzo A, Saccomano M, Napp J, Ellegaard M, Alves F, Novak I. Targeting of the P2X7 receptor in pancreatic cancer and stellate cells. Int J Cancer. 2016;139:2540-52 pubmed 出版商
  • 流式细胞仪; 小鼠; 图 2
  • 免疫细胞化学; 小鼠; 图 2
  • 免疫印迹; 小鼠; 图 2
Karmakar M, Katsnelson M, Dubyak G, Pearlman E. Neutrophil P2X7 receptors mediate NLRP3 inflammasome-dependent IL-1β secretion in response to ATP. Nat Commun. 2016;7:10555 pubmed 出版商
Wang A, Wong V, Lee P, Bui B, Dudczig S, Vessey K, et al. Retinal ganglion cell dysfunction in mice following acute intraocular pressure is exacerbated by P2X7 receptor knockout. Sci Rep. 2021;11:4184 pubmed 出版商
Lee D, Kim J. Protein disulfide isomerase-mediated S-nitrosylation facilitates surface expression of P2X7 receptor following status epilepticus. J Neuroinflammation. 2021;18:14 pubmed 出版商
Pegoraro A, Orioli E, De Marchi E, Salvestrini V, Milani A, Di Virgilio F, et al. Differential sensitivity of acute myeloid leukemia cells to daunorubicin depends on P2X7A versus P2X7B receptor expression. Cell Death Dis. 2020;11:876 pubmed 出版商
Benzaquen J, Dit Hreich S, Heeke S, Juhel T, Lalvee S, Bauwens S, et al. P2RX7B is a new theranostic marker for lung adenocarcinoma patients. Theranostics. 2020;10:10849-10860 pubmed 出版商
Qin J, Zhang X, Tan B, Zhang S, Yin C, Xue Q, et al. Blocking P2X7-Mediated Macrophage Polarization Overcomes Treatment Resistance in Lung Cancer. Cancer Immunol Res. 2020;8:1426-1439 pubmed 出版商
Brisson L, Chadet S, Lopez Charcas O, Jelassi B, Ternant D, Chamouton J, et al. P2X7 Receptor Promotes Mouse Mammary Cancer Cell Invasiveness and Tumour Progression, and Is a Target for Anticancer Treatment. Cancers (Basel). 2020;12: pubmed 出版商
Skarratt K, Gu B, Lovelace M, Milligan C, Stokes L, Glover R, et al. A P2RX7 single nucleotide polymorphism haplotype promotes exon 7 and 8 skipping and disrupts receptor function. FASEB J. 2020;34:3884-3901 pubmed 出版商
Feehan A, Zadina J. Morphine immunomodulation prolongs inflammatory and postoperative pain while the novel analgesic ZH853 accelerates recovery and protects against latent sensitization. J Neuroinflammation. 2019;16:100 pubmed 出版商
Carluccio M, Zuccarini M, Ziberi S, Giuliani P, Morabito C, Mariggio M, et al. Involvement of P2X7 Receptors in the Osteogenic Differentiation of Mesenchymal Stromal/Stem Cells Derived from Human Subcutaneous Adipose Tissue. Stem Cell Rev. 2019;: pubmed 出版商
Reigada D, Calder xf3 n Garc xed a A, Soto Catal xe1 n M, Nieto D xed az M, Mu xf1 oz Galdeano T, Del xc1 guila x, et al. MicroRNA-135a-5p reduces P2X7 -dependent rise in intracellular calcium and protects against excitotoxicity. J Neurochem. 2019;151:116-130 pubmed 出版商
Palygin O, Ilatovskaya D, Levchenko V, Klemens C, Dissanayake L, Williams A, et al. Characterization of purinergic receptor expression in ARPKD cystic epithelia. Purinergic Signal. 2018;14:485-497 pubmed 出版商
Kaczmarek Hájek K, Zhang J, Kopp R, Grosche A, Rissiek B, Saul A, et al. Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody. elife. 2018;7: pubmed 出版商
D Addio F, Vergani A, Potena L, Maestroni A, Usuelli V, Ben Nasr M, et al. P2X7R mutation disrupts the NLRP3-mediated Th program and predicts poor cardiac allograft outcomes. J Clin Invest. 2018;128:3490-3503 pubmed 出版商
Komalavilas P, Luo W, Guth C, Jolayemi O, Bartelson R, Cheung Flynn J, et al. Vascular surgical stretch injury leads to activation of P2X7 receptors and impaired endothelial function. PLoS ONE. 2017;12:e0188069 pubmed 出版商
Green J, Souilhol C, Xanthis I, Martinez Campesino L, Bowden N, Evans P, et al. Atheroprone flow activates inflammation via endothelial ATP-dependent P2X7-p38 signalling. Cardiovasc Res. 2017;: pubmed 出版商
Leduc Pessah H, Weilinger N, Fan C, Burma N, Thompson R, Trang T. Site-Specific Regulation of P2X7 Receptor Function in Microglia Gates Morphine Analgesic Tolerance. J Neurosci. 2017;37:10154-10172 pubmed 出版商
Albalawi F, Lu W, Beckel J, Lim J, McCaughey S, Mitchell C. The P2X7 Receptor Primes IL-1β and the NLRP3 Inflammasome in Astrocytes Exposed to Mechanical Strain. Front Cell Neurosci. 2017;11:227 pubmed 出版商
Bartlett R, Sluyter V, Watson D, Sluyter R, Yerbury J. P2X7 antagonism using Brilliant Blue G reduces body weight loss and prolongs survival in female SOD1G93A amyotrophic lateral sclerosis mice. Peerj. 2017;5:e3064 pubmed 出版商
Reigada D, Navarro Ruiz R, Caballero López M, Del Águila A, Muñoz Galdeano T, Maza R, et al. Diadenosine tetraphosphate (Ap4A) inhibits ATP-induced excitotoxicity: a neuroprotective strategy for traumatic spinal cord injury treatment. Purinergic Signal. 2017;13:75-87 pubmed 出版商
Migita K, Ozaki T, Shimoyama S, Yamada J, Nikaido Y, Furukawa T, et al. HSP90 Regulation of P2X7 Receptor Function Requires an Intact Cytoplasmic C-Terminus. Mol Pharmacol. 2016;90:116-26 pubmed 出版商
Elkhal A, Rodriguez Cetina Biefer H, Heinbokel T, Uehara H, Quante M, Seyda M, et al. NAD(+) regulates Treg cell fate and promotes allograft survival via a systemic IL-10 production that is CD4(+) CD25(+) Foxp3(+) T cells independent. Sci Rep. 2016;6:22325 pubmed 出版商
图像
图像 1 :
Alomone Labs APR-008 图像 1
Western blot analysis of rat brain membranes (lanes 1 and 5) and human K562 chronic myelogenous leukemia cell line (lanes 2 and 6) and Mouse WEHI-231 B cell lymphoma (lanes 3 and 7) and human HL-60  promyelocytic leukemia cell line (lanes 4 and 8):  - 1-4. Anti-P2X7 Receptor (extracellular) Antibody (#APR-008), (1:200).  5-8. Anti-P2X7 Receptor (extracellular) Antibody, preincubated with P2X7 Receptor (extracellular) Blocking Peptide (#BLP-PR008).
图像 2 :
Alomone Labs APR-008 图像 2
Expression of P2RX7 in rat pancreas - Immunohistochemical staining of rat pancreas  frozen section using Anti-P2X7 Receptor (extracellular) Antibody (#APR-008), (1:100), followed by mouse anti-rabbit-AlexaFluor-594 secondary antibody (red). Staining is present in endocrine cells of the Isle of Langerhans. Hoechst 33342 is used as the counterstain.
产品信息
SKU号 :
APR-008_1_25_mcl
ParentSKU :
APR-008
Taxonomy Node :
Primary Antibody>Extracellular>Non-Labeled
Action :
Insert
产品名称 :
抗P2X7受体(细胞外)抗体
产品描述 :
Anti-P2X7 Receptor (extracellular) Antibody (#APR-008) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, immunohistochemistry, live cell imaging, and immunocytochemistry applications. It has been designed to recognize P2X7 purinergic receptor from mouse, rat, and human samples.
Short Description :
A兔多克隆抗体 P2X7受体
Negative Control :
BLP-PR008
同义词 :
P2X7受体, 嘌呤受体P2X7, P2RX7, P2Z, ATP受体
Primary Category (Old) :
Ion Channels > Ligand-Gated Ion Channels > Purinergic (P2X) Receptors > Antibodies to P2X Receptors
标记/共轭标签 :
未共轭
应用 :
免疫细胞化学, IF, IFC, 免疫组化, LCI, 免疫印迹
宿主 :
兔
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
P2RX7
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Human, rat - identical; bovine - 14/17 amino acid residues identical.
靶标/背景信息 :
The P2X7 receptor is a member of the ionotropic P2X receptor family that is activated by ATP. To date, this family is composed of seven cloned receptor subtypes, named P2X1-P2X7. The different P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is found in cells of the immune system, particularly antigen presenting cells, and microglia. The P2X7 receptor mediates the release of proinflammatory cytokines, stimulation of transcription factors and may also have an important role in apoptosis.1-3 Different techniques have been used to characterize the P2X7 receptor. Most of them investigated pores, ion channels (electrophysiology) and membrane alterations (calcium microfluorometry, dye uptake, membrane depolarization and ion influx analysis). With the introduction of flow cytometry, it is now possible to analyze multiple cell parameters such as cell cycle, cell membrane alteration, calcium influx and cell phenotype.4
免疫原 :
合成肽
区域 :
细胞外
Loop :
Extracellular loop
序列 :
KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor
序列文本 :
Peptide KKGWMDPQSKGIQTGRC, corresponding to amino acid residues 136-152 of mouse P2X7 receptor (Accession Q9Z1M0). Extracellular loop.
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.8毫克/毫升
浓度 :
08毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
Q9Z1M0
Top Related :
APR-004, APR-002, APR-008-F, A-385, APR-024
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
IFC Legend :
Cell surface detection of P2X7 Receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: Cell surface detection of P2X7 Receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells: Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells +  Anti-P2X7 Receptor (extracellular) Antibody (#APR-008), (2.5μg) + goat-anti-rabbit-FITC.
LCI, ICC Legend :
Expression of P2RX7 in rat RBL cells - Cell surface detection of P2RX7 in intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-P2X7 Receptor (extracellular) Antibody (#APR-008), (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.
更多信息或购买 :
Alomone Labs 产品网页
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年，在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。