抗P2Y12受体抗体 | Alomone Labs APR-012 产品信息

产品简要

公司名称 :

Alomone Labs

产品类型 :

抗体

产品名称 :

抗P2Y12受体抗体

APR-012

规格 :

1 X 25 mcl

价格 :

425美元

克隆性 :

多克隆

宿主 :

domestic rabbit

共轭标签 :

未共轭

反应物种 :

人类, 小鼠, 大鼠

应用 :

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-石蜡切片

更多信息或购买 :

Alomone Labs 产品网页

文章摘录数: 14

出版应用/物种/样本/稀释	参考文献
免疫组化-石蜡切片; 小鼠; 1:200; 图 2j	Borges P, Waclawiak I, Georgii J, Fraga Junior V, Barros J, Lemos F, et al. Adenosine Diphosphate Improves Wound Healing in Diabetic Mice Through P2Y12 Receptor Activation. Front Immunol. 2021;12:651740 pubmed 出版商
免疫组化-石蜡切片; 人类; 1:200; 图 1	Walker D, Tang T, Mendsaikhan A, Tooyama I, Serrano G, Sue L, et al. Patterns of Expression of Purinergic Receptor P2RY12, a Putative Marker for Non-Activated Microglia, in Aged and Alzheimer's Disease Brains. Int J Mol Sci. 2020;21: pubmed 出版商
	Quek H, Cuní López C, Stewart R, Colletti T, Notaro A, Nguyen T, et al. ALS monocyte-derived microglia-like cells reveal cytoplasmic TDP-43 accumulation, DNA damage, and cell-specific impairment of phagocytosis associated with disease progression. J Neuroinflammation. 2022;19:58 pubmed 出版商
	Defaye M, Abdullah N, Iftinca M, Hassan A, Agosti F, Zhang Z, et al. Gut-innervating TRPV1+ Neurons Drive Chronic Visceral Pain via Microglial P2Y12 Receptor. Cell Mol Gastroenterol Hepatol. 2022;13:977-999 pubmed 出版商
	Ronzano R, Roux T, Thetiot M, Aigrot M, Richard L, Lejeune F, et al. Microglia-neuron interaction at nodes of Ranvier depends on neuronal activity through potassium release and contributes to remyelination. Nat Commun. 2021;12:5219 pubmed 出版商
	Banerjee A, Lu Y, Do K, Mize T, Wu X, Chen X, et al. Validation of Induced Microglia-Like Cells (iMG Cells) for Future Studies of Brain Diseases. Front Cell Neurosci. 2021;15:629279 pubmed 出版商
	Olotu C, Kiefmann M, Ronneburg C, Lehmensiek F, Cuvenhaus A, Meidl V, et al. Analysis of purine receptor expression and functionality in alveolar epithelial cells. Purinergic Signal. 2020;16:213-229 pubmed 出版商
	Alves M, Smith J, Engel T. Differential Expression of the Metabotropic P2Y Receptor Family in the Cortex Following Status Epilepticus and Neuroprotection via P2Y1 Antagonism in Mice. Front Pharmacol. 2019;10:1558 pubmed 出版商
	Alves M, de Diego García L, Conte G, Jimenez Mateos E, D Orsi B, Sanz Rodriguez A, et al. Context-Specific Switch from Anti- to Pro-epileptogenic Function of the P2Y1 Receptor in Experimental Epilepsy. J Neurosci. 2019;39:5377-5392 pubmed 出版商
	Zou L, Han X, Liu S, Gong Y, Wu B, Yi Z, et al. Baicalin Depresses the Sympathoexcitatory Reflex Induced by Myocardial Ischemia via the Dorsal Root Ganglia. Front Physiol. 2018;9:928 pubmed 出版商
	Yi Z, Xie L, Zhou C, Yuan H, Ouyang S, Fang Z, et al. P2Y12 receptor upregulation in satellite glial cells is involved in neuropathic pain induced by HIV glycoprotein 120 and 2',3'-dideoxycytidine. Purinergic Signal. 2018;14:47-58 pubmed 出版商
	Koessler J, Schwarz M, Weber K, Etzel J, Koessler A, Böeck M, et al. The role of adenosine diphosphate mediated platelet responsiveness for the stability of platelet integrity in citrated whole blood under ex vivo conditions. PLoS ONE. 2017;12:e0188193 pubmed 出版商
	Kamiyama M, Shirai T, Tamura S, Suzuki Inoue K, Ehata S, Takahashi K, et al. ASK1 facilitates tumor metastasis through phosphorylation of an ADP receptor P2Y12 in platelets. Cell Death Differ. 2017;24:2066-2076 pubmed 出版商
	Ribeiro Filho A, Buri M, Barros C, Dreyfuss J, Nader H, Justo G, et al. Functional and molecular evidence for heteromeric association of P2Y1 receptor with P2Y2 and P2Y4 receptors in mouse granulocytes. BMC Pharmacol Toxicol. 2016;17:29 pubmed 出版商

图像

图像 1 :

Western blot analysis of rat brain membranes (1 and 2) and human platelets (3 and 4): - 1,3. Anti-P2Y12 Receptor Antibody (#APR-012), (1:200). 2,4. Anti-P2Y12 Receptor Antibody, preincubated with P2Y12 Receptor Blocking Peptide (#BLP-PR012).

图像 2 :

Expression of P2RY12 in mouse brain - Immunohistochemical staining of mouse brain sections using Anti-P2Y12 Receptor Antibody (#APR-012), (red). The region shown in this picture is the entorhinal cortex in mouse brain. The P2Y12 positive fibers (arrows) run from the corpus callosum (triangles) into the deep layers of the entorhinal cortex. Sytox green is used as the counterstain.

产品信息

SKU号 :

APR-012_1_25_mcl

ParentSKU :

APR-012

Taxonomy Node :

Primary Antibody>Intracellular>Non-Labeled

Action :

Insert

产品名称 :

抗P2Y12受体抗体

产品描述 :

Anti-P2Y12 Receptor Antibody (#APR-012) is a highly specific antibody directed against an intracellular epitope of the human P2RY12. The antibody can be used in western blot, immunoprecipitation, indirect flow cytometry, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize P2RY12 from human, rat, and mouse samples.

Short Description :

A兔多克隆抗体P2Y12受体

Negative Control :

BLP-PR012

同义词 :

P2Y12受体, P2Y嘌呤受体12, P2RY12, ADP葡萄糖受体, 血小板ADP受体, HORK3

靶标名 :

P2Y12受体

Primary Category (Old) :

G-Protein Coupled Receptors > Purinergic (P2Y) Receptors > Antibodies to P2Y Receptors

标记/共轭标签 :

未共轭

应用 :

免疫细胞化学, IF, IFC, 免疫组化, 免疫沉淀, 免疫印迹

宿主 :

兔

克隆性 :

多克隆

抗体亚型 :

兔IgG

基因符号 :

P2RY12

反应物种 :

人类(试验);小鼠(试验);大鼠(试验)

同源 :

Rat, mouse - 16/18 amino acid residues identical.

靶标/背景信息 :

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y14.1-3 The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation. The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting.4 The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders.4

免疫原 :

合成肽

区域 :

intracellular

Loop :

Intracellular, 2nd cytoplasmic loop

序列 :

(C)KTTRPFKTSNPKNLLGAK, corresponding to amino acid residues 125-142 of human P2RY12

序列文本 :

Peptide (C)KTTRPFKTSNPKNLLGAK, corresponding to amino acid residues 125-142 of human P2RY12 (Accession Q9H244). Intracellular, 2nd cytoplasmic loop.

Primary Size (Size+Units) :

1 X 25 mcl

浓度 :

0.8毫克/毫升

浓度 :

08毫克/毫升

缓冲液 :

磷酸盐缓冲液(PBS)

pH值 :

pH值7.4

防腐剂 :

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Additive(s) :

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

格式(液体/冻干) :

冻干粉末

纯化来源(杂交瘤细胞, 腹水 :

Affinity purified on immobilized antigen

储存温度 :

-20°C

UniProt数据库 :

Q9H244

Top Related :

APR-020, APR-009, APR-004, APR-010, APR-002

储存和重组 :

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

包装规格 :

产品规格/规格 :

单位 :

mcl

标价美国[美元] :

425美元

更多信息或购买 :

Alomone Labs 产品网页

公司信息

Alomone Labs

Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201

info@alomone.com

http://www.alomone.com

972-2-587-2202

公司总部: 以色列

Alomone Labs公司成立于1989年，在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。