抗体

豚鼠抗P2Y12受体(细胞外)抗体

APR-020-GP

1 X 50 mcl

560美元

多克隆

豚鼠

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

APR-020-GP

Primary Antibody>Extracellular>Non-Labeled

Insert

豚鼠抗P2Y12受体(细胞外)抗体

Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP) is a highly specific antibody directed against an epitope of the human P2RY12. The antibody can be used in western blot and live imaging applications. It and has been designed to recognize P2RY12 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

A豚鼠多克隆抗体P2Y12受体

BLP-PR020

P2Y12受体, P2Y嘌呤受体12, P2RY12, ADP葡萄糖受体, 血小板ADP受体, HORK3

P2Y12受体

未共轭

免疫细胞化学, IF, 免疫组化, LCI, 免疫印迹

豚鼠

多克隆

豚鼠总数IgG

P2RY12

人类(试验);小鼠(试验);大鼠(试验)

Mouse – 12/13 amino acid residues identical; rat – 12/13 amino acid residues identical.

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y141-3.  The P2Y12 receptor is co-expressed with P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation. The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting4. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders4.

合成肽

细胞外

3rd extracellular loop

CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12

Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12 (Accession Q9H244). 3rd extracellular loop.

1 X 50 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

Q9H244

Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

1

50

mcl

560美元

Expression of P2RY12 in human glioblastoma (U-87 MG) cells - Cell surface detection of P2RY12 in intact living U-87 MG cells. A. Extracellular staining of cells using Guinea pig Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020-GP), (1:100) followed by goat anti-guinea pig-AlexaFluor-594 secondary antibody (red). DAPI was used as the counterstain (blue). B. Live view of the cells merged with P2Y12 staining.