抗体

抗P2Y12受体(细胞外)抗体

APR-020

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫细胞化学, 免疫组化-石蜡切片

APR-020

Primary Antibody>Extracellular>Non-Labeled

Insert

抗P2Y12受体(细胞外)抗体

Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020) is a highly specific antibody directed against an extracellular epitope of the human P2RY12. The antibody can be used in western blot, live cell imaging and indirect flow cytometry applications. It has been designed to recognize P2RY12 from human, rat and mouse samples.

A Rabbit Polyclonal Antibody to P2Y12 Receptor

BLP-PR020

P2Y12受体, P2Y嘌呤受体12, P2RY12, ADP葡萄糖受体, 血小板ADP受体, HORK3

P2Y12受体

G-Protein Coupled Receptors > Purinergic (P2Y) Receptors > Antibodies to P2Y Receptors

未共轭

免疫细胞化学, IF, IFC, LCI, 免疫印迹

兔

多克隆

兔IgG

P2RY12

Human (Tested);Rat (Tested);Mouse (Predicted)

Rat, mouse - 12/13 amino acid residues identical.

The P2Y receptors belong to the G-protein coupled receptor superfamily. They mediate the actions of the extracellular nucleotides (ATP, ADP, UTP and UDPA). Eight functional mammalian P2Y receptors have been described: P2Y1, P2Y2, P2Y4, P2Y6, P2Y11, P2Y12, P2Y13, and the UDP-glucose receptor, now renamed P2Y141-3.  The P2Y12 receptor is co-expressed with the P2Y1 receptor on platelets leading to shape change, aggregation, and rise in intracellular Ca2+ upon activation. The only other expression of P2Y12 was found in brain, according to results of reverse transcription-polymerase chain reaction and northern blotting4. The P2Y12 receptor has become a target for potential therapeutic drugs for the treatment of thromboembolism and other clotting disorders4.

合成肽

细胞外

3rd extracellular loop

CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12

Peptide CTAENTLFYVKES, corresponding to amino acid residues 270-282 of human P2RY12 (Accession Q9H244). 3rd extracellular loop.

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

Q9H244

APR-021, APR-012, APR-020-F, APR-024, AGP-098

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

1

25

mcl

425美元

Cell surface detection of P2RY12 in live intact human MEG-01 chronic myelogenous leukemia cells: Cell surface detection of P2RY12 in live intact human MEG-01 chronic myelogenous leukemia cells: Black line: Unstained cells. Green line: Cells + Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020), (5-10 µg/1x106 cells).

Expression of P2RY12 in rat RBL cells - Cell surface detection of P2RY12 in intact living rat basophilic leukemia (RBL) cells. A. Extracellular staining of cells using Anti-P2Y12 Receptor (extracellular) Antibody (#APR-020), (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells.