抗体

抗人类PAR1(F2R)(细胞外)-FITC抗体

APR-031-F

1 X 50 mcl

735美元

多克隆

domestic rabbit

异硫氰酸荧光素

APR-031-F

Primary Antibody>Extracellular>Labeled

Insert

抗人类PAR1(F2R)(细胞外)-FITC抗体

Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031) is a highly specific antibody directed against an epitope of human protease-activated receptor-1. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell flow cytometry applications. It has been designed to recognize PAR-1 from human samples. nAnti-Human PAR1 (F2R) (extracellular)-FITC Antibody (#APR-031-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct live cell flow cytometry.

A Rabbit Polyclonal Antibody to Protease-Activated Receptor-1 Conjugated to the Fluorescent dye FITC

RIC-001-F

PAR1(F2R), 蛋白酶激活受体1, PAR-1, 凝血酶受体, 凝血因子II受体, CF2R

蛋白酶激活受体1

G-Protein Coupled Receptors > Protease-Activated Receptors > Antibodies

荧光素异硫氰酸酯(异硫氰酸荧光素)

FC, LCI

兔

多克隆

兔IgG

F2R

人类(试验)

Monkey - identical.

Protease-activated receptor 1 (PAR-1) belongs to a family of four G protein-coupled receptors (PAR1-4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This results in the creation of a new N-terminal tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways.1-3 Different PARs are activated by different proteases. Hence, PAR-1 is activated by thrombin (and is in fact also known as the thrombin receptor), as are PAR-3 and PAR-4, while PAR-2 is activated by trypsin.1-3 PAR-1 can be also cleaved and activated by other proteases such as plasmin, Factor Xa, cathepsin G, and others. The intramolecular nature of PAR activation and the continuous presence of the tethered ligand that cannot diffuse away imply the existence of several mechanisms for the rapid termination of PAR signaling. Indeed, following receptor activation, there is rapid phosphorylation of the C-terminal end of the receptor, followed by receptor internalization and degradation. In addition, several proteases can cleave away the tethered ligand, thereby “disarming” the PAR.1-3 PAR-1 signals through several G proteins including Gαq, Gαi, and Gα12/13, resulting in the activation of several transduction pathways including intracellular Ca2+ mobilization, Rho and Rac signaling, and MAPK activation.1-3 PAR-1 is expressed in several cell types including platelets, leukocytes, vascular endothelial cells, gastrointestinal epithelial cells, myocytes, and neurons. The best studied physiological function of PAR-1 is its involvement in the coagulation cascade. Thrombin, the preeminent ligand of PAR-1, activates the receptor on the surface of platelets, hence inducing platelet aggregation, granular secretion, and procoagulant activity. PAR-1 also plays a crucial role in vascular ontogenesis. Accordingly, PAR-1 knockout mice show bleeding at multiple sites and usually die at mid-gestation.1-3 PAR-1 also plays important roles in tumor growth and metastasis. PAR-1 is upregulated in several human cancers as are several proteases such as plasmin and matrix metalloproteases (MMPs) that act as PAR-1 ligands, thereby creating an autocrine loop. PAR-1 activation in cancer cells transmits mitogenic signals through the activation of the erk1/2 pathway and is involved in tumor spread via its pro-angiogenic activity.4

合成肽

细胞外

细胞外, N末端

(C)KNESGLTEYRLVSINK, corresponding to amino acid residues 61-76 of human PAR-1

Peptide (C)KNESGLTEYRLVSINK, corresponding to amino acid residues 61-76 of human PAR-1 (Accession P25116). Extracellular, N terminal.

1 X 50 mcl

1毫克/毫升

1毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P25116

Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

50

mcl

735美元