产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗人类PAR1(F2R)(细胞外)抗体
目录 :
APR-031
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类
应用 :
免疫印迹, 免疫组化, 免疫细胞化学
更多信息或购买 :
图像
图像 1 :
Alomone Labs APR-031 图像 1
Western blot analysis of human promyelocytic leukemia HL-60 (lanes 1 and 4), human T-cell leukemia Jurkat (lanes 2 and 5), and chronic myelogenous leukemia K562 (lanes 3 and 6) cell line lysates: - 1-3. Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031), (1:200).4-6. Anti-Human PAR1 (F2R) (extracellular) Antibody, preincubated with Human PAR1/F2R (extracellular) Blocking Peptide (#BLP-PR031).
图像 2 :
Alomone Labs APR-031 图像 2
Expression of PAR-1 in normal human breast and human breast carcinoma - Immunohistochemical staining of paraffin-embedded human breast sections using Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031), (1:100). PAR-1 staining is highly specific for epithelium-derived cells. A. In the normal resting breast, epithelial cells of the mammary ducts are visible using Histofine (pink). B. The breast carcinoma contains epithelium-derived malignant cells stained with DAB (brown). Hematoxilin is used as the counterstain.
图像 3 :
Alomone Labs APR-031 图像 3
Western blot analysis of human colon cancer HT-29 (lanes 1 and 3) and Colo-205 (lanes 2 and 4) cell line lysates: - 12. Anti-Human PAR1 (F2R) (extracellular) Antibody(#APR-031) (1:200).34. Anti-Human PAR1 (F2R) (extracellular) Antibody preincubated with the negative control antigen.
产品信息
SKU号 :
APR-031_1_25_mcl
ParentSKU :
APR-031
Taxonomy Node :
Primary Antibody>Extracellular>Non-Labeled
Action :
Insert
产品名称 :
抗人类PAR1(F2R)(细胞外)抗体
产品描述 :
Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031) is a highly specific antibody directed against an epitope of human protease-activated receptor-1. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and live cell flow cytometry applications. It has been designed to recognize PAR-1 from human samples.
Short Description :
A兔多克隆抗体蛋白酶激活受体1
Negative Control :
BLP-PR031
同义词 :
PAR1(F2R), 蛋白酶激活受体1, PAR-1, 凝血酶受体, 凝血因子II受体, CF2R
靶标名 :
蛋白酶激活受体1
Primary Category (Old) :
G-Protein Coupled Receptors > Protease-Activated Receptors > Antibodies
标记/共轭标签 :
未共轭
应用 :
免疫细胞化学, IF, IFC, 免疫组化, LCI, 免疫印迹
宿主 :
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
F2R
反应物种 :
人类(试验)
同源 :
Monkey - identical.
靶标/背景信息 :
Protease-activated receptor 1 (PAR-1) belongs to a family of four G protein-coupled receptors (PAR1-4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This results in the creation of a new N-terminal tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways.1-3 Different PARs are activated by different proteases. Hence, PAR-1 is activated by thrombin (and is in fact also known as the thrombin receptor), as are PAR-3 and PAR-4, while PAR-2 is activated by trypsin.1-3 PAR-1 can be also cleaved and activated by other proteases such as plasmin, Factor Xa, cathepsin G, and others. The intramolecular nature of PAR activation and the continuous presence of the tethered ligand that cannot diffuse away imply the existence of several mechanisms for the rapid termination of PAR signaling. Indeed, following receptor activation, there is rapid phosphorylation of the C-terminal end of the receptor, followed by receptor internalization and degradation. In addition, several proteases can cleave away the tethered ligand, thereby “disarming” the PAR.1-3 PAR-1 signals through several G proteins including Gaq, Gai, and Ga12/13, resulting in the activation of several transduction pathways including intracellular Ca2+ mobilization, Rho and Rac signaling, and MAPK activation.1-3 PAR-1 is expressed in several cell types including platelets, leukocytes, vascular endothelial cells, gastrointestinal epithelial cells, myocytes, and neurons. The best studied physiological function of PAR-1 is its involvement in the coagulation cascade. Thrombin, the preeminent ligand of PAR-1, activates the receptor on the surface of platelets, hence inducing platelet aggregation, granular secretion, and procoagulant activity. PAR-1 also plays a crucial role in vascular ontogenesis. Accordingly, PAR-1 knockout mice show bleeding at multiple sites and usually die at mid-gestation.1-3 PAR-1 also plays important roles in tumor growth and metastasis. PAR-1 is upregulated in several human cancers as are several proteases such as plasmin and matrix metalloproteases (MMPs) that act as PAR-1 ligands, thereby creating an autocrine loop. PAR-1 activation in cancer cells transmits mitogenic signals through the activation of the erk1/2 pathway and is involved in tumor spread via its pro-angiogenic activity.4
免疫原 :
合成肽
区域 :
细胞外
Loop :
细胞外, N末端
序列 :
(C)KNESGLTEYRLVSINK, corresponding to amino acid residues 61-76 of human PAR-1
序列文本 :
Peptide (C)KNESGLTEYRLVSINK, corresponding to amino acid residues 61-76 of human PAR-1 (Accession P25116). Extracellular, N terminal.
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.8毫克/毫升
浓度 :
08毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P25116
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
IFC Legend :
Cell surface detection of PAR-1 by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: Cell surface detection of PAR-1 by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells + Anti-Human PAR1 (F2R) (extracellular) Antibody (#APR-031), (2.5μg) + goat-anti-rabbit-FITC.
LCI, ICC Legend :
Expression of PAR-1 in human prostate PC-3 cell line - Cell surface detection of PAR-1 in human prostate PC-3 cell line. A. Live intact PC-3 cells were stained with Anti-Human PAR1 (F2R)(extracellular) Antibody (#APR-031), (1:50), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (red staining). Nuclei were visualized using the cell-permeable dye Hoechst 33342 (blue). B. Live view of the same field as A.
更多信息或购买 :
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年,在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。