抗体

抗PAR2(F2RL1)抗体

APR-032

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

APR-032

Primary Antibody>Intracellular>Non-Labeled

Insert

抗PAR2(F2RL1)抗体

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat protease-activated receptor-2. Anti-PAR2 (F2RL1) Antibody (#APR-032) can be used in western blot, immunohistochemistry and and immunocytochemistry applications. It has been designed to recognize PAR-2 from rat, mouse and human samples.

A兔多克隆抗体蛋白酶激活受体

BLP-PR032

PAR2 (F2RL1), Protease-activated receptor-2, PAR-2, Thrombin receptor-like 1, Coagulation factor II receptor-like 1, GPR11

蛋白酶激活受体

G-Protein Coupled Receptors > Protease-Activated Receptors > Antibodies

未共轭

免疫细胞化学, IF, 免疫组化, 免疫印迹

兔

多克隆

兔IgG

F2RL1

人类(试验);小鼠(试验);大鼠(试验)

Mouse - 13/15 amino acid residues identical; human 11/15 amino acid residues residues identical.

Protease-activated receptor 2 (PAR-2) belongs to a family of four G protein-coupled receptors (PAR1-4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This results in the creation of a new N-terminal tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways.1-3 Different PARs are activated by different proteases. Hence, PAR-1 is activated by thrombin, as are PAR-3 and PAR-4.1-3 PAR-2 is the only PAR family receptor that is activated by trypsin and not by thrombin. PAR-2 can be also cleaved and activated by other proteases such as tryptase, membrane-type serine protease 1, protease 3, and others.1-3 The intramolecular nature of PAR activation and the continuous presence of the tethered ligand that cannot diffuse away imply the existence of several mechanisms for the rapid termination of PAR signaling. Indeed, following receptor activation, there is rapid phosphorylation of the C-terminal end of the receptor, followed by receptor internalization and degradation. In addition, several proteases can cleave away the tethered ligand, thereby “disarming” the PAR.1-3 PAR-2-mediated intracellular signaling has not been clearly elucidated, but activators of PAR-2 induce generation of IP3 and mobilization of intracellular Ca2+. Activation of the ERK1/2 and NF-kB intracellular pathways following PAR-2 activation has been also described.1-3 Tissue distribution of PAR-2 is wide with the highest expression levels found in pancreas, small intestine, liver and kidney. In addition, PAR-2 expression was observed in dorsal root ganglion neurons, smooth muscle cells and endothelial cells. The physiological role of PAR-2 is not clearly understood, however studies using PAR-2 knockout mice have suggested roles in the cardiovascular, pulmonary and gastrointestinal systems. Several studies suggest that PAR-2 plays a central role in inflammatory diseases and nociceptive pain transduction; hence PAR-2 blockers have been proposed to be useful in the therapeutic control of inflammation and pain.1-3

合成肽

intracellular

Intracellular, C-terminus

(C)KRMQISLTSNKFSRK, corresponding to amino acid residues 368-382 of rat PAR-2

Peptide (C)KRMQISLTSNKFSRK, corresponding to amino acid residues 368-382 of rat PAR-2 (Accession Q63645). Intracelluar, C- terminus.

1 X 25 mcl

0.6毫克/毫升

06毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

Q63645

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元

Expression of PAR-2 in human prostate carcinoma cell line - Immunocytochemical staining of paraformaldehyde-fixed and permeabilized human PC-3 cells. A. Cells were stained with Anti-PAR2 (F2RL1) Antibody (#APR-032), (1:500), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody (orange). B. Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue). C. Merge of A and B.