抗体

抗PAR4(F2RL3)(细胞外)抗体

APR-034

1 X 50 mcl

530美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

APR-034

Primary Antibody>Extracellular>Non-Labeled

Insert

抗PAR4(F2RL3)(细胞外)抗体

Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034) is a highly specific antibody directed against an extracellular epitope of human protease-activated receptor-4. The antibody can be used in western blot analysis, immunohistochemical, immunocytochemical and flow cytometry applications. It has been designed to recognized PAR-4 from human, mouse and rat samples.

A兔多克隆抗体蛋白酶激活受体4

BLP-PR034

PAR4 (F2RL3), Protease-activated receptor-4, PAR-4, Coagulation factor II receptor-like 3, Thrombin receptor-like 3

蛋白酶激活受体4

G-Protein Coupled Receptors > Protease-Activated Receptors > Antibodies

未共轭

免疫细胞化学, IF, IFC, 免疫组化, LCI, 免疫印迹

兔

多克隆

兔IgG

F2RL3

人类(试验);小鼠(试验);大鼠(试验)

Rat, mouse - identical.

Protease-activated receptor 4 (PAR-4) belongs to a family of four G protein-coupled receptors (PAR1-4) that are activated as a result of proteolytic cleavage by certain serine proteases, hence their name. In this novel modality of activation, a specific protease cleaves the PAR receptor within a defined sequence in its extracellular N-terminal domain. This results in the creation of a new N-terminal tethered ligand, which subsequently binds to a site in the second extracellular loop of the same receptor. This binding results in the coupling of the receptor to G proteins and in the activation of several signal transduction pathways.1-3 Different PARs are activated by different proteases. Hence, PAR-4 is activated by both thrombin and trypsin whereas PAR-1 and PAR-3 are activated only by thrombin and PAR-2 is activated only by trypsin.1-3 PAR-4 can be also cleaved and activated by other proteases such as cathepsin G. The intracellular signaling mechanisms mediated by PAR-4 activation are not completely elucidated but they involve calcium mobilization downstream of phospholipase Cβ through the Gαq pathway.1-3 Tissue distribution of PAR-4 is very broad with the highest expression levels found in lung, testis, pancreas and small intestine. In addition, PAR-4 expression was observed in platelets, megakaryocytes and leukocytes. Studies with platelets derived form PAR-4 knockout mice have established an essential role for PAR-4 in thrombin-induced platelet activation. PAR-4 is likely involved in other physiological functions such as regulation of gastrointestinal motility and regulation of vascular endothelial cell function.1-3

合成肽

细胞外

1st extracellular loop

(C)HLRGQRWPFGEAA(S)R, corresponding to amino acid residues 136-150 of human PAR-4

Peptide (C)HLRGQRWPFGEAA(S)R, corresponding to amino acid residues 136-150 of human PAR-4 (Accession Q96RI0). Cys 149 was replaced with Ser. 1st extracellular loop.

1 X 50 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

Q96RI0

APR-031, APR-034-F

Reconstitution: 1 X 50 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

50

mcl

530美元

Cell surface detection of PAR-4 by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: Cell surface detection of PAR-4 by indirect flow cytometry in live intact human MEG-01 megakaryocytic leukemia cells: Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells + Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034), (2.5μg) + goat-anti-rabbit-FITC.

Expression of PAR-4 in a human breast adenocarcinoma cell line - Cell surface detection of PAR-4 in a human breast adenocarcinoma cell line. A. Live intact human MCF-7 cells were stained with Anti-PAR4 (F2RL3) (extracellular) Antibody (#APR-034), (1:50), followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells. C. Nuclei were visualized with the cell-permeable dye Hoechst 33342 (blue).