抗体

抗前列腺素E受体EP2(PTGER2)(细胞外)抗体

APR-064

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

APR-064

Primary Antibody>Extracellular>Non-Labeled

Insert

抗前列腺素E受体EP2(PTGER2)(细胞外)抗体

Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize EP2 receptor from rat, mouse, and human samples.

A兔多克隆抗体类前列腺素EP2受体

BLP-PR064

前列腺素E受体EP2(PTGER2), 前列腺素E2受体EP2亚类, PGE2受体EP2亚类, 类前列腺素EP2受体

类前列腺素EP2受体

G-Protein Coupled Receptors > Prostanoid Receptors > Antibodies

未共轭

IF, IFC, 免疫组化, LCI, 免疫印迹

兔

多克隆

兔IgG

PTGER2

人类(试验);小鼠(试验);大鼠(试验)

Mouse - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical.

Prostaglandin E2 (PGE2) is involved in a number of physiological and pathophysiological events in many tissues throughout the body1. The physiological actions of PGE2 are mediated through its interaction with cell surface prostaglandin E receptors. There are three pharmacologically defined subtypes of the EP receptor, EP1, EP2, and EP3, and these subtypes are suggested to be different in their signal transduction2. These receptors belong to the G-protein coupled receptor (GPCR) superfamily. Like all members they have seven transmembrane domains with an extracellular N-terminal tail and an intracellular C-terminus. The EP2 receptor is expressed in the vasculature, the gastrointestinal tract, kidney and also in the ciliary muscles of the eye3. PGE2 is known to play a central role in the pathophysiology of inflammation in synergy with other proinflammatory mediators. PGE2 inhibits the function and the proliferation of T cells and the histamine release from mast cells by increasing the intracellular level of cAMP4. The EP2 subtype is thought to be in part responsible for vasodilation, oedema formation, hyperanalgesia, modulation of the immune system, and the breakdown of bone and cartilage associated with disorders such as rheumatoid arthritis5.

合成肽

细胞外

3rd extracellular loop

(C)DETSSRKEKWDLR, corresponding to amino acid residues 287-299 of rat prostanoid EP2 receptor

Peptide (C)DETSSRKEKWDLR, corresponding to amino acid residues 287-299 of rat prostanoid EP2 receptor (Accession Q62928). 3rd extracellular loop.

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

Q62928

APR-063, APR-065

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 × g 5 min).

1

25

mcl

425美元

Cell surface detection of EP2 receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells Cell surface detection of EP2 receptor by indirect flow cytometry in live intact human THP-1 monocytic leukemia cells Black line: Cells. Red line: Cells + goat-anti-rabbit-FITC. Green line: Cells + Anti-Prostaglandin E Receptor EP2/PTGER2 (extracellular) Antibody (#APR-064), (2.5μg) + goat-anti-rabbit-FITC.