抗体

抗PSD-95抗体

APZ-009

1 X 25 mcl

425美元

多克隆

domestic rabbit

未共轭

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学

APZ-009

Primary Antibody>Intracellular>Non-Labeled

Insert

抗PSD-95抗体

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope human PSD-95. Anti-PSD-95 Antibody (#APZ-009) can be used in western blot and immunohistochemistry applications. It has been designed to recognize PSD-95 from mouse, rat and human samples.

A兔多克隆抗体PSD-95

BLP-PZ009

PSD-95, Postsynaptic density protein 95, Discs large homolog 4, DLG4, Synapse-associated protein 90, SAP-90, Tax interaction protein 15

PSD-95

Neural Signaling > PDZ-Domain Scaffolding Proteins > Antibodies

未共轭

IF, 免疫组化, 免疫印迹

兔

多克隆

兔IgG

DLG4

人类(试验);小鼠(试验);大鼠(试验)

Rat, mouse – identical.

The postsynaptic density (PSD) is a membrane-associated protein specialized in postsynaptic signal transduction and processing. There are four PSD family members classified according to their molecular weight, including, PSD-95, PSD-93, synaptic associated proteins 97 kDa and 102 kDa. PSD-95 interacts with both ionotropic and metabotropic glutamate receptors via protein–protein interactions and plays a role in their precise assembly and spatial organization as well as coupling of these receptors to downstream signaling events1. PSD-95 contains a SH3 domain, a GK domain and three PDZ domains. The PDZ domains 1 and 2 of PSD-95 are separated by only a few residues, creating a rigid bond that may restrain interdomain flexibility. These two PDZ domains can have distinct or overlapping target-binding proteins. In contrast, PDZ3 of PSD-95 is located at the carboxyl terminal end of the protein and has a set of interacting proteins that are distinct from PDZ domains 1 and 2. The N-palmitoylation of PSD-95 at Cys-3 and Cys-5 residues is essential for enhanced synaptic responses and the trafficking of PSD-95 to the plasma membrane2. With its numerous domains, PSD-95 is often referred to as a scaffold protein, which localizes and traffics various receptors, cell adhesion molecules, ion channels, kinases and phosphatases to the synaptic membrane. PSD-95 also interacts with a variety of adaptor and cytoskeleton proteins, molecular motors, as well as protein synthesis machinery3. PSD-95 is found at the postsynaptic membrane of excitatory synapses4. PSD-95 null mice have severe learning defects and exhibit both facilitation of long-term potentiation and disruption of long-term depression5.

合成肽

intracellular

Intracellular, between the SH3 and the GK-like domains.

(C)KDWGSSSGSQGRED, corresponding to amino acid residues 505-518 of human PSD-95

Peptide (C)KDWGSSSGSQGRED, corresponding to amino acid residues 505-518 of human PSD-95 (Accession P78352). Intracellular, between the SH3 and the GK-like domains.

1 X 25 mcl

0.8毫克/毫升

08毫克/毫升

磷酸盐缓冲液(PBS)

pH值7.4

1% BSA, 0.05% NaN3 (% of reconstituted antibody)

Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

冻干粉末

Affinity purified on immobilized antigen

-20°C

P78352

AIP-005, AGP-021, AGP-129, ANR-014, ANR-013

Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

1

25

mcl

425美元