产品简要
公司名称 :
Alomone Labs
产品类型 :
抗体
产品名称 :
抗ASIC1抗体
目录 :
ASC-014
规格 :
1 X 25 mcl
价格 :
425美元
克隆性 :
多克隆
宿主 :
domestic rabbit
共轭标签 :
未共轭
反应物种 :
人类, 小鼠, 大鼠
应用 :
免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 免疫组化-冰冻切片
更多信息或购买 :
Alomone Labs 产品网页
文章摘录数: 9
出版应用/物种/样本/稀释参考文献
  • 免疫细胞化学; 大鼠; 图 2c
  • 免疫印迹; 大鼠; 1:200; 图 2a
Hu Y, Mi X, Huang C, Wang H, Song J, Shu Q, et al. Multiple H+ sensors mediate the extracellular acidification-induced [Ca2+]i elevation in cultured rat ventricular cardiomyocytes. Sci Rep. 2017;7:44951 pubmed 出版商
  • 免疫组化-冰冻切片; 小鼠; 1:200; 图 6
Ferenczi E, Vierock J, Atsuta Tsunoda K, Tsunoda S, Ramakrishnan C, Gorini C, et al. Optogenetic approaches addressing extracellular modulation of neural excitability. Sci Rep. 2016;6:23947 pubmed 出版商
Li Y, Tian Y, Wu Y, Xu Y, Zhang P, Sha J, et al. Upregulation of Spinal ASIC1 and NKCC1 Expression Contributes to Chronic Visceral Pain in Rats. Front Mol Neurosci. 2020;13:611179 pubmed 出版商
Liu X, Liu C, Ye J, Zhang S, Wang K, Su R. Distribution of Acid Sensing Ion Channels in Axonal Growth Cones and Presynaptic Membrane of Cultured Hippocampal Neurons. Front Cell Neurosci. 2020;14:205 pubmed 出版商
Nomura K, Hiyama T, Sakuta H, Matsuda T, Lin C, Kobayashi K, et al. [Na+] Increases in Body Fluids Sensed by Central Nax Induce Sympathetically Mediated Blood Pressure Elevations via H+-Dependent Activation of ASIC1a. Neuron. 2018;: pubmed 出版商
Stephan G, Huang L, Tang Y, Vilotti S, Fabbretti E, Yu Y, et al. The ASIC3/P2X3 cognate receptor is a pain-relevant and ligand-gated cationic channel. Nat Commun. 2018;9:1354 pubmed 出版商
Trac P, Thai T, Linck V, Zou L, Greenlee M, Yue Q, et al. Alveolar nonselective channels are ASIC1a/?-ENaC channels and contribute to AFC. Am J Physiol Lung Cell Mol Physiol. 2017;312:L797-L811 pubmed 出版商
Song N, Guan R, Jiang Q, Hassanzadeh C, Chu Y, Zhao X, et al. Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception. Sci Rep. 2016;6:38777 pubmed 出版商
Wu Y, Qin D, Yang H, Fu H. Evidence for the Participation of Acid-Sensing Ion Channels (ASICs) in the Antinociceptive Effect of Curcumin in a Formalin-Induced Orofacial Inflammatory Model. Cell Mol Neurobiol. 2017;37:635-642 pubmed 出版商
图像
图像 1 :
Alomone Labs ASC-014 图像 1
Western blot analysis of rat brain membranes: - 1. Anti-ASIC1 Antibody (#ASC-014), (1:200).   2. Anti-ASIC1 Antibody, preincubated with ASIC1 Blocking Peptide (#BLP-SC014).
图像 2 :
Alomone Labs ASC-014 图像 2
Expression of ASIC1 in rat brain - Immunohistochemical staining of rat globus pallidus using Anti-ASIC1 Antibody (#ASC-014). A. Parvalbumin (PV) positive neurons are shown (green). B. Neurons with typically enmeshed dendritic trees stain intensely for ASIC1 (red). C. Double labeling with mouse anti-Parvalbumin (green) reveals strong co-localization of PV with ASIC1.
图像 3 :
Alomone Labs ASC-014 图像 3
Western blot analysisof rat brain membranes: - 1.Anti-ASIC1 Antibody (#ASC-014) (1:200). 2. Anti-ASIC1 Antibody preincubated with the control peptide antigen.
产品信息
SKU号 :
ASC-014_1_25_mcl
ParentSKU :
ASC-014
Taxonomy Node :
Primary Antibody>Intracellular>Non-Labeled
Action :
Insert
产品名称 :
抗ASIC1抗体
产品描述 :
Anti-ASIC1 Antibody (#ASC-014) is an antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunoprecipitation, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize ASIC1 from rat, human, and mouse samples, and recognizes both ASIC1 isoforms.
Short Description :
A Rabbit Polyclonal Antibody to ASIC1 Channel
Negative Control :
BLP-SC014
同义词 :
ASIC1, Acid-sensing ion channel 1, Amiloride-sensitive brain sodium channel, BNaC2, ACCN2
靶标名 :
ASIC1 Channel
Primary Category (Old) :
Ion Channels > Na+ Channels > Acid-Sensing Ion Channels (ASIC) > Antibodies to ASICs
标记/共轭标签 :
未共轭
应用 :
免疫细胞化学, IF, 免疫组化, 免疫沉淀, 免疫印迹
宿主 :
兔
克隆性 :
多克隆
抗体亚型 :
兔IgG
基因符号 :
ASIC1
反应物种 :
人类(试验);小鼠(试验);大鼠(试验)
同源 :
Human - identical; mouse - 19/20 amino acid residues identical.
靶标/背景信息 :
ASIC1 is a member of a family of Na+ channels that are activated by external protons. The family includes four additional members: ASIC2, ASIC3, ASIC4 and ASIC5. The ASICs are in fact part of a larger superfamily named degenerin/epithelial Na+ channels (DEG/ENaC) and share with it the same basic characteristics: two transmembrane spanning domains, a large extracellular domain and short intracellular N- and C-termini. There are two recognized splice variants of the ASIC1 gene that differ on their N-termini, ASIC1a and ASIC1b that have different tissue distributions and functions. ASIC1 responds to a decrease in extracellular pH with an inward cation current that is quickly inactivated despite the continuous presence of protons in the medium. Recently, ASIC1 has been implicated in cognitive processes such as learning and memory.
免疫原 :
合成肽
区域 :
intracellular
Loop :
Intracellular, C-terminus
序列 :
CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1
序列文本 :
Peptide CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 of rat ASIC1 (Accession P55926). Intracellular, C-terminus.
Primary Size (Size+Units) :
1 X 25 mcl
浓度 :
0.8毫克/毫升
浓度 :
08毫克/毫升
缓冲液 :
磷酸盐缓冲液(PBS)
pH值 :
pH值7.4
防腐剂 :
1% BSA, 0.05% NaN3 (% of reconstituted antibody)
Additive(s) :
Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.
格式(液体/冻干) :
冻干粉末
纯化来源(杂交瘤细胞, 腹水 :
Affinity purified on immobilized antigen
储存温度 :
-20°C
UniProt数据库 :
P55926
Top Related :
ASC-012, ASC-018, ASC-031, AGP-053, STP-200
储存和重组 :
Reconstitution: 1 X 25 mcl double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C, as long as it is in its original form, it can last a minimum of 3 years this way. The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
包装规格 :
1
产品规格/规格 :
25
单位 :
mcl
标价美国[美元] :
425美元
更多信息或购买 :
Alomone Labs 产品网页
公司信息
Alomone Labs
Jerusalem BioPark (JBP), Hadassah Ein Kerem
P.O. Box 4287
Jerusalem 9104201
info@alomone.com
http://www.alomone.com
972-2-587-2202
公司总部: 以色列
Alomone Labs公司成立于1989年，在离子通道、GPCR及神经信号通路研究等领域占据全球领先地位。