产品简要
公司名称 :
American Research Products
产品类型 :
抗体
产品名称 :
抗弹性蛋白
目录 :
03-61013
规格 :
200微升
价格 :
129.00美元
克隆性 :
单克隆
宿主 :
小鼠
共轭标签 :
nonconjugated
反应物种 :
人类
应用 :
免疫印迹, 酶联免疫吸附测定
更多信息或购买 :
产品信息
目录代码 :
03-61013
产品名称 :
抗弹性蛋白
宿主物种 :
小鼠
克隆性 :
单克隆
共轭 :
纯化
产品类型 :
小鼠单克隆
规格 :
200微升
标价 :
129.00美元
克隆 :
VIM3B4
抗体亚型 :
IgG2a
产品描述 :
The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to vimentin. Polypeptide reacting: Mr 57 000 intermediate filament protein (vimentin) of mesenchymal cells. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma.The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).
免疫原 :
Vimentin (purified from bovine lens)
应用摘要 :
Immunoblotting, ELISA, immunofluorescence microscopy, immunohistochemistry. Suitable for frozen and paraffin-embedded tissue and cytological material
储存 :
2-8°C for immediate use, or at -20°C (aliquot)
一般参考 :
Franke, W.W. and Moll, R. (1987).:Differentiation 36: 145-163Heid, H.W. et al. (1988). Differentiation 37: 137-157Jahn, L.et al. (1987). Differentiation 36: 234-254Kasper, M.et al. (1989). Differentiation 40: 207-214Kasper, M.et al. (1989). Histochemistry 93: 93-103Mol, I. & Moll, R. (1991) Differentiation 40: 207-214Rungger-Braendle, E. et al. (1989) J Cell Biol. 109: 705-716Gomi, H. et al. (1995) Neuron 14: 29-41Demirkesen , C. et al. (1995) J. Cutan Pathol 22: 518-535Hermann, H. et al. (1993) J. Mol. Biol 234: 99-113Rogers, KR et al. (1995) Eur J Cell Biol 66: 136-150Bohn, W. et al. (1992) Exp. Cell Res 201: 1-7Hermann, H. et al. (1992) J. Mol Biol 223: 637-650
更多信息或购买 :
公司信息
American Research Products
135 Beaver Street, Suite 204
Waltham, MA 02452
info@arp1.com
www.arp1.com
800-832-2611 781-373-2182
公司总部: 美国