抗体

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 460-480), (C-Term) antibody

ABIN117897

0.2毫升

489.5美元

多克隆

兔

nonconjugated

人类, 小鼠, 大鼠

免疫印迹, 免疫组化, 免疫细胞化学, 免疫沉淀, 酶免疫法, 免疫组化-冰冻切片

anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 460-480), (C-Term) antibody

兔

多克隆

AKT1/PKB

Primary

0.2毫升

489.5美元

11686294,11356119

Immunohistochemistry. Rabbit anti-AKT was used at a 1:1,000 dilution to detect AKT by immunohistochemistry. ,.In panel (A) normal colon tissue is stained. Panel (B) shows tumor tissue. Tissue was formalin-fixed and paraffin embedded.

AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase

Synthetic peptide corresponding to the C-terminus (460-480) of Human, Rat and Mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

氨基酸460-480, C末端

Chicken,Human,Mouse (Murine),Rat (Rattus)

0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % Sodium Azide

85 mg/mL (by refractometry)

Enzyme Immunoassay (EIA),Immunofluorescence (IF),Immunohistochemistry (Frozen Sections) (IHC (fro)),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)),Immunoprecipitation (IP),Western Blotting (WB)

P31749