ELISA/assay

增殖细胞核抗原酶联免疫吸附试剂盒(人类)(OKCD02760)

OKCD02760

96 Wells

Application Kit

酶联免疫吸附试剂盒

增殖细胞核抗原酶联免疫吸附试剂盒(人类)(OKCD02760)

96 Wells

增殖细胞核抗原

细胞周期蛋白, 增殖细胞核抗原

NM_002592.2

NP_002583.1

P12004

人类

Store as indicated in product manual.

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Assay Methodology: Quantitative Sandwich ELISA

0.055 ng/mL

This assay has high sensitivity and excellent specificity for detection of Proliferating Cell Nuclear Antigen (PCNA). No significant cross-reactivity or interference between Proliferating Cell Nuclear Antigen (PCNA) and analogues was observed.

Wet Ice

2-8C