小鼠 单克隆 (DE2B4)
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 免疫沉淀
文章摘录数: 3
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定, 免疫细胞化学, 免疫沉淀
文章摘录数: 3
Published customer image: . Mouse anti Human beta amyloid antibody, clone DE2B4 used for the evaluation of amyloid deposition in brain lysates by dot and western blotting. Image caption: . Soluble and insoluble TPC6A aggregates are present in the hippocampi of nondemented human controls and AD samples (A) Frozen postmortem AD and control human hippocampal tissues were homogenized by sonication. After centrifugation, the insoluble pellets were further extracted and analyzed by filter retardation assay. Western blotting was then carried out using specific antibodies for TPC6A (pan-specific), TIAF1, Tyr33-phosphorylated WWOX (p-WWOX), NFT and Aβ. No significant difference was shown in TPC6A or TIAF1 aggregation for samples between younger nondemented controls (14 samples per group; total 3 groups; 42 to 76 years old) and older AD patients (24 samples per group; total 4 groups; 71 to 90 years old). C = control; A or AD = Alzheimer's disease. (B) The supernatants of the aforementioned samples were analyzed by 7% non-reducing SDS-PAGE. Western blotting analyses show the presence of soluble aggregates of TPC6A and Aβ in the representative protein preparations. Sample numbers are shown in each panel. Molecular weight markers: 170, 130, 95, 72 kDa. (C) Hippocampal tissue sections of APP/PS1-transgenic mice were stained with aliquots of TPC6A pan-specific antiserum and then with an Alexa Fluor 488-conjugated secondary antibody. Fluoro-Jade C was used for staining degenerative neurons, and nuclei stained with DAPI. TPC6A aggregates are localized in the degenerative neurons. A representative tissue section is shown. Scale bar: 50 μm. In negative controls, the sections were stained with the secondary antibody only, followed by staining with Fluoro-Jade C and DAPI. (D) Immuno-intensity was measured and normalized to negative controls (mean ± standard deviation, n = 5; **p Oncotarget. 2015 Feb 28;6(6):3578-89 .
规格: 0.1毫克
价格: 357美元
至产商
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹
文章摘录数: 1
反应物种: 人类
应用: 免疫印迹
文章摘录数: 1
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
domestic rabbit 多克隆
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
反应物种: 人类
应用: 免疫印迹, 酶联免疫吸附测定
Published customer image: . Rabbit anti Beta amyloid antibody (AHP677) used for the demonstration of total amyloid load in mouse brain by immunohistochemistry on free floating tissue sections. Image caption: . Total A load is reduced following intracranial administration of intact anti-A antibody and deglycosylated anti-A antibody. Panels B, D, and F show total A immunostaining in the left (untreated) hippocampal regions of 20 mo. old APP transgenic mice. Panels A, C, and E show total A staining in right hippocampal regions of 20 mo. old APP transgenic mice receiving intracranial injection of control antibody (panel A) or anti-A C-terminal antibody (2H6; panel C), or deglycosylated anti-A C-terminal antibody (de-2H6; panel E). Magnification = 40×, scale bar = 50 mm. Panel G shows quantification of the A load as the ratio of injected (right) side to uninjected (left) side for both the hippocampal and frontal cortical injection sites * indicates P 0.05 compared to mice injected with control IgG. From: Carty NC, Wilcock DM, Rosenthal A, Grimm J, Pons J, Ronan V, Gottschall PE, Gordon MN, Morgan D. Intracranial administration of deglycosylated C-terminal-specific anti-Abeta antibody efficiently clears amyloid plaques without activating microglia in amyloid-depositing transgenic mice. J Neuroinflammation. 2006 May 10;3:11 .
规格: 0.1毫升
价格: 477美元
至产商
小鼠 单克隆 (MAb1.1)
反应物种: 人类
应用: 酶联免疫吸附测定
反应物种: 人类
应用: 酶联免疫吸附测定
Staining of paraffin embedded human Alzheimer’s disease brain tissue with Mouse anti Human beta amyloid (aa37-42) (MCA5930GA) and detected using a Goat anti Mouse IgG:HRP secondary antibody. Top image: 5x magnification showing diffuse plaques in Alzheimer’s disease brain. Bottom left image: 20x magnification showing diffuse plaques in Alzheimer’s disease brain. Bottom right image: Alzheimer’s disease brain stained with an unrelated antibody as negative control
规格: 0.1毫克
价格: 357美元
至产商
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